Lai, Meng Yee and Suppiah, Jeyanthi and Thayan, Ravindran and Ismail, Ilyiana and Mustapa, Nur Izati and Soh, Tuan Suhaila Tuan and Hassan, Afifah Haji and Peariasamy, Kalaiarasu M. and Lee, Yee Leng and Lau, Yee Ling (2022) RNA purification-free detection of SARS-CoV-2 using reverse transcription loop-mediated isothermal amplification (RT-LAMP). Tropical Medicine and Health, 50 (1). ISSN 1348-8945, DOI https://doi.org/10.1186/s41182-021-00396-y.
Full text not available from this repository.Abstract
Background: Current diagnosis of SARS-CoV-2 infection relies on RNA purification prior to amplification. Typical extraction methods limit the processing speed and turnaround time for SARS-CoV-2 diagnostic testing. Methods: Here, we applied reverse transcription loop-mediated isothermal amplification directly onto human clinical swabs samples to amplify the RNA from SARS-CoV-2 swab samples after processing with chelating resin. Results: By testing our method on 64 samples, we managed to develop an RT-LAMP assay with 95.9 sensitivity (95 CI 86 to 99.5) and 100 specificity (95 CI 78.2–100). Conclusion: The entire process including sample processing can be completed in approximately 50 min. This method has promising potential to be applied as a fast, simple and inexpensive diagnostic tool for the detection of SARS-CoV-2. © 2022, The Author(s).
| Item Type: | Article |
|---|---|
| Funders: | Prototype Research Grant Scheme (PRGS) from the Ministry of Education, Malaysia [Grant No: PR001-2020B (PRGS/2/2020/SKK09/UM/02/1)] |
| Uncontrolled Keywords: | Virus RNA; Article; Controlled study; Geobacillus stearothermophilus; Human; Isothermal amplification; Loop mediated isothermal amplification; Nasopharyngeal swab; Nonhuman; Oropharyngeal swab; Reverse transcription loop mediated isothermal amplification; RNA purification; Sensitivity and specificity; Severe acute respiratory syndrome coronavirus 2; Turnaround time; Virus nucleocapsid |
| Subjects: | R Medicine > R Medicine (General) R Medicine > RV Botanic, Thomsonian, and eclectic medicine |
| Divisions: | Faculty of Medicine > Parasitology Deparment |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 28 Sep 2023 12:46 |
| Last Modified: | 28 Sep 2023 12:46 |
| URI: | http://eprints.um.edu.my/id/eprint/43188 |
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