Elemental sulphur and alkali elutable melanin detected in oral melanosis and malignant melanoma by energy-filtering transmission electron microscopy

Nagai, N. and Lee, Y.J. and Nagaoka, N. and Gunduz, M. and Nakano, K. and Nojima, T. and Tsujigiwa, H. and Gunduz, E. and Siar, C.H. and Nagatsuka, H. (2002) Elemental sulphur and alkali elutable melanin detected in oral melanosis and malignant melanoma by energy-filtering transmission electron microscopy. Journal of Oral Pathology & Medicine, 31 (8). pp. 481-487. ISSN 0904-2512, DOI https://doi.org/10.1034/j.1600-0714.2002.00010.x.

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Abstract

Background: The morphology and contents of melanosomes are important features for differentiating melanocyte-derived melanotic lesions such as melanosis and malignant melanoma. Methods: In this study, we attempted to elucidate the structure of melanin and sulphur content in oral melanosis and malignant melanomas by ultrastructural analysis. Results: In oral melanosis, the essential pathological findings were overproduction of eumelanin and discharge of melanin into keratinocytes. In malignant melanoma in situ, pleomorphic and ellipsoid abnormal melanosomes with an increase in sulphur content and alkali elution rate were detected. In invasive malignant melanoma, the irregular ellipsoid and spheroid melanosomes existing either as discrete bodies or compound melanosomes with furtherly increased sulphur content and alkali elution were detected. Conclusions: Our findings suggest that abnormal melanosome morphology and high sulphur content are predictive markers for assessment of early or precancerous melanotic lesions and malignant melanoma.

Item Type:	Article
Funders:	UNSPECIFIED
Additional Information:	ISI Document Delivery No.: 594KC Times Cited: 6 Cited Reference Count: 12 Cited References: Banfalvi T, 2000, EUR J CLIN INVEST, V30, P900, DOI 10.1046/j.1365-2362.2000.00710.x CURRAN RC, 1976, J PATHOL, V119, P135, DOI 10.1002/path.1711190303 Donois E, 1998, ANAL QUANT CYTOL, V20, P275 Donois E, 1998, J INVEST DERMATOL, V111, P422, DOI 10.1046/j.1523-1747.1998.00313.x DRZEWIECKI KT, 1979, SCAND J PLAST RECONS, V13, P321 Horikoshi Takashi, 1992, Journal of Dermatology (Tokyo), V19, P809 INAZU M, 1993, J INVEST DERMATOL, V100, pS172, DOI 10.1038/jid.1993.9 Mizuhira V, 1998, ACTA HISTOCHEM CYTOC, V31, P217 MORISHIMA T, 1993, SURG TODAY, V23, P580 PROTA G, 1995, PIGM CELL RES, V8, P153, DOI 10.1111/j.1600-0749.1995.tb00657.x Vincensi MR, 1998, MELANOMA RES, V8, P53, DOI 10.1097/00008390-199802000-00009 Wimmer I, 1997, CANCER RES, V57, P5073 Nagai, N Lee, YJ Nagaoka, N Gunduz, M Nakano, K Nojima, T Tsujigiwa, H Gunduz, E Siar, CH Nagatsuka, H Blackwell munksgaard Copenhagen
Uncontrolled Keywords:	Melanosome melanoma melanosis sulphur TEM stereologic image-analysis 5-s-cysteinyldopa pheomelanin eumelanin cells
Subjects:	R Medicine > RK Dentistry
Divisions:	Faculty of Dentistry > Dept of Oral Pathology & Oral Medicine & Periodontology
Depositing User:	Ms Nursyafiqah Abd Malek
Date Deposited:	09 Jan 2013 01:04
Last Modified:	09 Jan 2013 01:04
URI:	http://eprints.um.edu.my/id/eprint/4288

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