Kazmi, Syeda Kahkashan and Kayani, Hammad Afzal and Raziq, Marium and Naz, Sheeba and Khan, Saifullah and Kabir, Nurul (2022) Protoplast isolation and regeneration from leaves and nucellar embryos of Kinnow mandarin (Citrus reticulata blanco). Pakistan Journal of Botany, 54 (4). pp. 1359-1364. ISSN 0556-3321, DOI https://doi.org/10.30848/PJB2022-4(27).
Full text not available from this repository.Abstract
Citrus is one of the most dominant horticultural fruit crops in the world. For the genetic improvement of Kinnow mandarin (Citrus reticulate blanco), protoplast isolation and somatic hybridization strategy is suitable. In this study, Kinnow mandarin protoplasts were isolated to get single species regeneration. Protoplasts were obtained from explants like In vitro and field grown leaves and nucellar embryos. Enzymatic protoplast isolation was performed and then yield, and viability testing was done. After initial testing of protoplast their regeneration was achieved. Nucellar embryos gave best regeneration but protoplasts from leaves were unable to regenerate. This study showed that 16 hours incubation was found best for optimum viable protoplasts yield of 5.4 x 10(4) protoplasts/gm FW (fresh weight) from nucellar embryos and 17.3 x 10(4) protoplasts/gm FW (fresh weight) of non-viable protoplasts yield from leaves.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Protoplast isolation; Root stock improvement; Enzymatic hydrolysis; Nucellar embryos; Protoplast regeneration; Yield testing; Viability testing |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 25 Aug 2023 06:43 |
| Last Modified: | 25 Aug 2023 06:43 |
| URI: | http://eprints.um.edu.my/id/eprint/42805 |
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