Teh, Cindy Shuan Ju and Yap, Polly Soo Xi and Zulkefli, Noorfatin Jihan and Subramaniam, Prasanna and Sit, Pik San and Kong, Zhi Xian and Chong, Chun Wie and Vellasamy, Kumutha Malar and Mariappan, Vanitha and Ponnampalavanar, Sasheela and Vadivelu, Jamuna (2022) Overview of the distribution of Burkholderia pseudomallei sequence types and the Emergence of sequence type 1342 in Malaysia. Transboundary And Emerging Diseases, 69 (2). pp. 477-484. ISSN 1865-1674, DOI https://doi.org/10.1111/tbed.14005.
Full text not available from this repository.Abstract
Burkholderia pseudomallei, a Gram-negative bacterial pathogen that causes melioidosis, is of public health importance in endemic areas including Malaysia. An investigation of the molecular epidemiology links of B. pseudomallei would contribute to better understanding of the clonal relationships, transmission dynamics and evolutionary change. Multilocus sequence typing (MLST) of 45 clinical B. pseudomallei isolates collected from sporadic melioidosis cases in Malaysia was performed. In addition, a total of 449 B. pseudomallei Malaysian strains submitted to the MLST database from 1964 until 2019 were included in the temporal analysis to determine the endemic sequence types (STs), emergence and re-emergence of ST(s). In addition, strain-specific distribution was evaluated using BURST tool. Genotyping of 45 clinical strains was resolved into 12 STs, and the majority were affiliated with ST46 (n = 11) and ST1342 (n = 7). Concomitantly, ST46 was the most prevalent ST in Malaysia, which was first reported in 1964. All the Malaysian B. pseudomallei strains were resolved into 76 different STs with 36 of them uniquely present only in Malaysia. ST1342 was most closely related to ST1034, in which both STs were unique to Malaysia and first isolated from soil samples in Pahang, a state in Malaysia. The present study revealed a high diversity of B. pseudomallei in Malaysia. Localized evolution giving rise to the emergence of new STs was observed, suggesting that host and environmental factors play a crucial role in the evolutionary changes in B. pseudomallei.
| Item Type: | Article |
|---|---|
| Funders: | University of Malaya Research Grant (UMRG) (Grant No. RP013B-13HTM), Ministry of Higher Education (MOHE), Malaysia, under the High Impact Research (HIR)-MOHE Project (Grant No. E000013-20001) |
| Uncontrolled Keywords: | Burkholderia pseudomallei; melioidosis; multilocus sequence typing; sequence type |
| Subjects: | Q Science > QR Microbiology R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 08 Oct 2023 11:31 |
| Last Modified: | 08 Oct 2023 13:10 |
| URI: | http://eprints.um.edu.my/id/eprint/42466 |
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