Vinnie-Siow, Wei Yin and Tan, Tiong Kai and Low, Van Lun and Teoh, Yong Bin and Prakash, Batah Kunalan and Sivanandam, Sinnadurai and Sharma, Reuben Sunil Kumar and Lim, Yvonne Ai-Lian (2022) Integration of Microscopic, Serologic and Molecular Techniques for Detection of Filarial Parasites in Dogs in Malaysia. Acta Parasitologica, 67 (1). pp. 468-475. ISSN 1230-2821, DOI https://doi.org/10.1007/s11686-021-00490-5.
Full text not available from this repository.Abstract
Purpose Canine filariosis in domestic dogs caused by several species of filarids is an emerging vector-borne disease and the spread of this disease remains a global veterinary and public health concern. However, information regarding these filarids and their epidemiological patterns remains scarce in Malaysia. The present study aimed to determine the infection rate and associated risk factors of filarial parasites in dogs in Malaysia. Methods A total of 399 dog blood samples were collected from veterinary hospitals and animal shelters in Malaysia to determine the infection rate and associated risk factors via a combination of microscopic, serologic and molecular diagnostic techniques. Results Two species of canine filariae identified in this study were Dirofilaria immitis (6.5%) and Brugia pahangi (1.3%), and their infections were associated with cross breed, medium size and short hair (p < 0.05). Conclusions A new pair of primers was developed to complement the recovery of the 12S rRNA gene fragment of filarial parasites. This study represents the first molecular evidence of B. pahangi in dogs in Malaysia.
| Item Type: | Article |
|---|---|
| Funders: | Gasing Veterinary Hospital, Higher Institution Centre of Excellence [Grant No:MO002-2019], Universiti Malaya [Grant No:PG098-2016A] |
| Uncontrolled Keywords: | Brugia pahangi; Canine filariosis; Dirofilaria immitis; Malaysia |
| Subjects: | Q Science > Q Science (General) R Medicine > R Medicine (General) |
| Divisions: | Faculty of Medicine > Parasitology Deparment Deputy Vice Chancellor (Research & Innovation) Office > Tropical Infectious Diseases Research and Education Centre |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 05 Oct 2023 07:10 |
| Last Modified: | 05 Oct 2023 07:10 |
| URI: | http://eprints.um.edu.my/id/eprint/42404 |
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