Roebuck, Margaret M. and Jamal, Juliana and Lane, Brian and Wood, Amanda and Santini, Alasdair and Wong, Pooi-Fong and Bou-Gharios, George and Frostick, Simon P. (2022) Cartilage debris and osteoarthritis risk factors influence gene expression in the synovium in end stage osteoarthritis. Knee, 37. pp. 47-59. ISSN 0968-0160, DOI https://doi.org/10.1016/j.knee.2022.05.001.
Full text not available from this repository.Abstract
Background: Gene expression in healthy synovium remains poorly characterised. Thus, synovial functional activity changes associated with osteoarthritis (OA) are difficult to define. This study sought to identify differentially expressed genes (DEG) of end-stage OA and assess the influence of OA risk factors on these DEG. Methods: Anonymised patient clinical data and x-ray images were analysed. Osteoarthritic and non-osteoarthritic patients with soft tissue or traumatic knee injuries were matched for body mass index (BMI) and sex. Tissue samples were partitioned for immunocytochem-istry (IHC) and microarray analysis. Multiple bioinformatics applications were utilised to determine changes in functional and canonical pathway activation. Results: Age, disease-modifying injections and hypertension were confounding factors between patient groups. Inflammation was present in all tissues. Cartilage debris and inflam-matory aggregates were noted in many osteoarthritic patient tissues. IHC and expression analyses revealed upregulation of synoviolin 1 (SYVN1) in osteoarthritic synovium. Significant differential expression was noted in 2084 genes. Osteoarthritic synovium displayed a significant upregulation of 95% of DEG coding for proteins, relative to non-osteoarthritic synovium tissues. Unfolded protein response (UPR)-related genes were upreg-ulated in osteoarthritic synovium; gene expression of molecules within many canonical pathways including protein ubiquitination and UPR pathways was modified by BMI and sex. Conclusions: The synovium of all three pathologies exhibited elements of an inflammatory response. Cartilage debris, age, BMI and sex influence DEG of osteoarthritic synovium. UPR pathway is the top deregulated canonical pathway identified in osteoarthritic synovium regardless of BMI and sex, while typical OA-associated inflammatory and matrix gene responses were minimal. (c) 2022 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Funders: | University of Malaya Dual PhD Program, Fundamental Research Grant Scheme of The Ministry of Higher Education Malaysia [Grant No:FRGS/1/2018/SKK08/UM/02/26], Fundamental Research Grant Scheme of The Ministry of Higher Education Malaysia [Grant No:FP011-2018A], University of Liverpool, Liverpool, UK [Grant No:11424], University of Liverpool, Liverpool, UK [Grant No:40424604] |
| Uncontrolled Keywords: | Osteoarthritis; Arthroplasty; Obesity; Synovium; Microarray |
| Subjects: | R Medicine > RD Surgery |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 17 Oct 2023 04:15 |
| Last Modified: | 17 Oct 2023 04:15 |
| URI: | http://eprints.um.edu.my/id/eprint/41938 |
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