Tan, Choo Hock and Oh, Angeline Mei Feng and Wong, Kin Ying and Liew, Jia Lee and Tan, Nget Hong and Tan, Kae Yi (2022) On characterizing the Red-headed Krait (Bungarus flaviceps) venom: Decomplexation proteomics, immunoreactivity and toxicity cross-neutralization by hetero-specific antivenoms. Comparative Biochemistry and Physiology D-Genomics & Proteomics, 43. ISSN 1744-117X, DOI https://doi.org/10.1016/j.cbd.2022.101006.
Full text not available from this repository.Abstract
The Red-headed Krait (Bungarus flaviceps) is a medically important venomous snake species in Southeast Asia, while there is no specific antivenom available for its envenoming. This study investigated the venom composition through a decomplexation proteomic approach, and examined the immunoreactivity as well as crossneutralization efficacy of two hetero-specific krait antivenoms, Bungarus candidus Monovalent Antivenom (BcMAV) and Bungarus fasciatus Monovalent Antivenom (BfMAV), against the venom of B. flaviceps from Peninsular Malaysia. A total of 43 non-redundant proteoforms belonging to 10 toxin families were identified in the venom proteome, which is dominated by phospholipases A2 including beta-bungarotoxin lethal subunit (56.20 % of total venom proteins), Kunitz-type serine protease inhibitors (19.40 %), metalloproteinases (12.85 %) and three-finger toxins (7.73 %). The proteome varied in quantitative aspect from the earlier reported Indonesian (Sumatran) sample, suggesting geographical venom variation. BcMAV and BfMAV were immunoreactive toward the B. flaviceps venom, with BcMAV being more efficacious in immunological binding. Both antivenoms cross-neutralized the venom lethality with varying efficacy, where BcMAV was more potent than BfMAV by \~13 times (normalized potency: 38.04 mg/g vs. 2.73 mg/g, defined as the venom amount completely neutralized by one-gram antivenom protein), supporting the potential utility of BcMAV for para-specific neutralization against B. flaviceps venom
| Item Type: | Article |
|---|---|
| Funders: | Universiti Malaya [Grant no. BKS003-2020] |
| Uncontrolled Keywords: | Snakebite envenoming; Neglected tropical disease; Venomics; Monovalent antivenom |
| Subjects: | R Medicine > RM Therapeutics. Pharmacology |
| Divisions: | Faculty of Medicine > Department of Molecular Medicine Faculty of Medicine > Department of Pharmacology |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 03 Jun 2025 08:51 |
| Last Modified: | 03 Jun 2025 08:51 |
| URI: | http://eprints.um.edu.my/id/eprint/41850 |
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