Rehiman, Siti Hajar and Lim, Siong Meng and Lim, Fei Tieng and Chin, Ai-Vyrn and Tan, Maw Pin and Kamaruzzaman, Shahrul Bahyah and Ramasamy, Kalavathy and Abdul Majeed, Abu Bakar (2022) Fibrinogen isoforms as potential blood-based biomarkers of Alzheimer's disease using a proteomics approach. International Journal of Neuroscience, 132 (10). pp. 1014-1025. ISSN 0020-7454, DOI https://doi.org/10.1080/00207454.2020.1860038.
Full text not available from this repository.Abstract
Objective: Alzheimer's disease (AD), the commonest form of dementia which is characterized by progressive decline in cognitive function, can only be definitively diagnosed after death. Although biomarkers may aid diagnosis, currently available AD biomarkers, which are predominantly based on cerebrospinal fluid and neuroimaging facilities, are either invasive or costly. Blood-based biomarkers for AD diagnosis are highly sought after due to its practicality at the clinic. This study was undertaken to determine the differential protein expression in plasma amongst Malaysian AD, mild cognitive impairment (MCI) and non-AD individuals. Methods: A proteomic approach which utilized two-dimensional differential in gel electrophoresis (2 D DIGE) was performed for blood samples from 15 AD, 14 MCI and 15 non-AD individuals. Results: Mass spectrometry (MS)-based protein identification via MALDI ToF/ToF showed that fibrinogen-beta-chain (spot 64) and fibrinogen-gamma-chain (spot 91) with differential expression ratio >1.5 were significantly upregulated (p < 0.05) in AD patients when compared to non-AD individuals. Further data analysis using Pearson correlation found that the upregulated fibrinogen-gamma-chain was weakly but significantly (p < 0.05) and inversely correlated with cognitive decline. Conclusion: Fibrinogen isoforms may play important roles in the vascular pathology of AD as well as neuroinflammation. As such, fibrinogen appears to be a promising blood-based biomarker for AD. Further validation of the present findings in larger population is now warranted.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Higher Education (MOHE) Malaysia (Grant No: UiTM RMI/FRGS 5/3 (61/2012) & UiTM-[600-RMC/LRGS 5/3 (002/2019)]) |
| Uncontrolled Keywords: | Alzheimer's disease; Blood-based biomarkers; Proteomics; 2D DIGE; Fibrinogen-β -chain; fibrinogen-γ -chain |
| Subjects: | R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry |
| Divisions: | Faculty of Medicine > Medicine Department |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 06 Nov 2023 02:58 |
| Last Modified: | 06 Nov 2023 02:58 |
| URI: | http://eprints.um.edu.my/id/eprint/41517 |
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