Nazari, V. Mansoureh and Mahmood, Syed and Shah, Amin Malik and Al-Suede, Fouad Saleh Resq (2022) Suppression of melanoma growth in a murine tumour model using orthosiphon stamineus benth. extract loaded in ethanolic phospholipid vesicles (spherosome). Current Drug Metabolism, 23 (4). pp. 317-328. ISSN 1389-2002, DOI https://doi.org/10.2174/1389200223666220416215129.
Full text not available from this repository.Abstract
Orthosiphon stamineus Benth (O.S) is a traditional south-east Asian herb. The extract of O.S is used in the formulation of ethanolic nanolipid vesicle system to have considerable potential for tumour therapeutics. Methods: The research objective is to develop and characterise the anticancer and antiangiogenic effect of O.S extract in the form of nano-ethanolic spherosomes (ESP) using phospholipids in melanoma. Spherosomes formulation of O.S was developed using the thin-film re-hydration method and converted to gel using Acrypol 1%. The formulations were subjected to optimisation and physical-chemical characterisations like particle size, surface charge, DSC, FTIR, and TEM. Cytotoxicity of O.S and ESP was studied using an endothelial cell line (EA. hy926). Furthermore, anti-melanoma effect of O.S spherosome gel was studied in albino mice after topical administration. Results: ESP-6 with the ratio of extract (O.S): cholesterol: phospholipid (1: 6: 0.5) showed the highest entrapment efficiency (80.56 +/- 0.84%) using ultraviolet spectroscopy. In-vivo permeation/penetration studies revealed deeper absorption of ESP-6 compared to a hydroethanolic gel of O.S. In-vitro and in vivo anti-melanoma studies demonstrated the significant tumour-suppressing effect of ESP-6 on murine melanoma. Percentage inhibition of tumour growth by O.S and ESP-6 at 3000 mg/kg showed to be 63.98 +/- 7.86% and 87.76 +/- 7.90%, respectively. Conclusion: Spherosome vesicles were developed with a smooth surface. The results demonstrated that O.S extract showed no toxicity when tested on the endothelial cell line. O.S loaded in spherosomes has the potential to lower the growth of melanoma in mice. The spherosomes loaded with O.S do not promote tumour growth or act as antiangio-genetic in melanoma.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Orthosiphon stamineus; Spherosomes; Angiogenesis; Melanoma; Anti-tumour; Spectroscopy |
| Subjects: | Q Science > QD Chemistry R Medicine R Medicine > RM Therapeutics. Pharmacology |
| Divisions: | Faculty of Pharmacy |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 25 Sep 2023 03:09 |
| Last Modified: | 25 Sep 2023 03:09 |
| URI: | http://eprints.um.edu.my/id/eprint/41471 |
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