Putra, Sharifah Shahira Syed and Basirun, Wan Jefrey and Elgharbawy, Amal A. M. and Hayyan, Adeeb and Hayyan, Maan and Mohammed, Mohammed Alhaji (2022) Nanocellulose and natural deep eutectic solvent as potential biocatalyst system toward enzyme immobilization. Molecular Catalysis, 528. ISSN 2468-8231, DOI https://doi.org/10.1016/j.mcat.2022.112422.
Full text not available from this repository.Abstract
This study reports the immobilization of Candida Rugosa lipase (CRL) onto nanocellulose (NC) extracted from almond shells using p-toluenesulfonic acid (PTSA) and sulfuric acid (ASS) with sugar-based natural deep eutectic solvent (NADES1a) as a biocatalyst system. The properties of both immobilized lipases were studied and compared to the free enzyme counterpart. Under optimized conditions (2 h, 40 degrees C and pH 7.0), the immobilized CRL-PTSA-NADES1a and CRL-ASS-NADES1a gave a maximum specific activity of 4.9 U mg(-1) and 6.57 U mg(-1), respectively, compared to the free CRL (4.52 U mg(-1)). Both immobilized CRL showed better thermal stability, high catalytic activity and reusability up to 7 consecutive cycles. The half-life of the immobilized lipase was 14 similar to 16 days greater than free lipase (27 days). The Brunauer-Emmett-Teller (BET) surface area of NC-ASS (20.76 m(2) g(-1)) is higher compared to NC-PTSA (4.81 m(2) g(-1)). The functional groups and morphology of the free and immobilized CRL were further determined by Fourier transformed infrared (FTIR) and scanning electron microscopy (SEM). These findings revealed that the immobilized CRL onto NCs and NADES1a as green materials and solvent, respectively had higher lipase immobilization efficiency and stability for the treatment of food contaminants in oils in order to satisfy increasing commercial demands in the oil industry.
| Item Type: | Article |
|---|---|
| Funders: | Universiti Malaya |
| Uncontrolled Keywords: | Candida Rugosa lipase; Nanotechnology; Green solvent; Biocatalysis; Oil industry |
| Subjects: | Q Science > QD Chemistry |
| Divisions: | Faculty of Science |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 13 Sep 2023 08:03 |
| Last Modified: | 13 Sep 2023 08:03 |
| URI: | http://eprints.um.edu.my/id/eprint/41212 |
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