Nageib, Adilah Mohamed and Jameel, Ahmad Tariq and Ahmad, Farah (2022) Kinetic studies of immobilized wheat-based alpha-naphthyl acetate esterase in the detection of organophosphate pesticides. Chemical Engineering & Technology, 45 (11, SI). pp. 2088-2095. ISSN 0930-7516, DOI https://doi.org/10.1002/ceat.202100406.
Full text not available from this repository.Abstract
Organophosphate pesticides could inhibit the activity of alpha-naphthyl acetate esterase (ANAE), which could be the fundamental for biosensor applications. k-carrageenan was used for immobilization of ANAE extracted from wheat-based sources. The activity of ANAE was assessed for optimum activity at a range of temperature and pH. Enzyme kinetic studies were performed using Michaelis-Menten kinetic parameters from Lineweaver-Burk, Langmuir, and Eddie-Hofstee. The optimization studies showed that the relative activity of the enzyme remained high, even above 50 degrees C. k(d) obtained was very low, which indicates the high sensitivity of ANAE towards pesticides. The kinetic reaction of ANAE showed the promising application of biosensors in the detection of pesticide.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Higher Education Malaysia through Fundamental Research Grant Scheme (FRGS) [FRGS/1/2017/TK02/UIAM/02/4] |
| Uncontrolled Keywords: | Alpha-naphthyl acetate esterase; Biosensors; Carrageenan; Enzyme immobilization; Wheat |
| Subjects: | T Technology > TP Chemical technology |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 27 Sep 2023 04:36 |
| Last Modified: | 27 Sep 2023 04:36 |
| URI: | http://eprints.um.edu.my/id/eprint/40810 |
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