AbuBakar, Sazaly and Azmi, A. and Misbah, S. and Chang, L.Y. (2002) Antigenic cell associated Dengue 2 Virus proteins detected in Vitro using Dengue fever patients Sera. Malaysian Journal of Pathology, 24 (1). pp. 29-36. ISSN 0126-8635,
Full text not available from this repository.Abstract
At least three major antigenic dengue 2 virus proteins were recognized by pooled dengue fever patients' sera in infected Aedes albopictus (C6/36) mosquito cells. Dengue virus envelope (E), premembrane (PrM) and non-structural protein 1 (NS 1) dimer were detected beginning on day 3 postinfection in both the cell membrane and cytosolic fractions. Using the patients' sera, the presence of antigenic intermediate core protein (C)-PrM and NS1-non-structural protein 2a (NS2a) in the cytoplasmic fraction of dengue 2 virus infected cells was revealed. The presence of a approximately 92 and approximately 84 kDa NS 1 dimer in the membrane (NS 1m) and cytosolic (NS 1c) fractions of C6/36 cells, respectively, was also recognized. Using individual patient's serum, it was further confirmed that all patients' sera contained antibodies that specifically recognized E, NS 1 and PrM present in the dengue 2 virus-infected cell membrane fractions, suggesting that these glycosylated virus proteins were the main antigenic proteins recognized in vivo. Detection of dengue 2 virus C antibody in some patients further suggested that C could be antigenic if presented in vivo.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Additional Information: | Department of Medical Microbiology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA |
| Uncontrolled Keywords: | Dengue |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | miss munirah saadom |
| Date Deposited: | 12 Nov 2012 01:33 |
| Last Modified: | 13 Feb 2019 08:03 |
| URI: | http://eprints.um.edu.my/id/eprint/3950 |
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