Xiong, Chuan and Zhu, Yu and Luo, Qiang and Phan, Chia Wei and Huo, Yujie and Li, Ping and Li, Qiang and Jin, Xin and Huang, Wenli (2023) Neuroprotective effects of a novel peptide from Lignosus rhinocerotis against 6-hydroxydopamine-induced apoptosis in PC12 cells by inhibiting NF-kappa B activation. Food Science & Nutrition, 11 (5). pp. 2152-2165. ISSN 2048-7177, DOI https://doi.org/10.1002/fsn3.3050.
Full text not available from this repository.Abstract
According to previous studies, oxidative stress is a leading cause of dopaminergic neuron death and may contribute to the pathogenesis of Parkinson's disease (PD). In the current study, we used chromatography of gel filtration to identify a novel peptide (Lignosus rhinocerotis peptide LRP]) from the sclerotium of Lignosus rhinocerotis (Cooke) Ryvarden. Its neuroprotective effect was evaluated using an in vitro PD model constructed by 6-hydroxydopamine (6-OHDA)-stimulated to apoptosis in PC12 cells. The molecular weight of LRP is determined as 1532 Da and the secondary structure is irregular. The simple amino acid sequence of LRP is Thr-Leu-Ala-Pro-Thr-Phe-Leu-Ser-Ser-Leu-Gly-Pro-Cys-Leu-Leu. Notably, LRP has the ability to significantly boost the viability of PC12 cells after exposure to 6-OHDA, as well as enhance the cellular activity of antioxidative enzymes like superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px). LRP also lowers the level of malondialdehyde (MDA), decreases the activation performance of Caspase-3, and reduces 6-OHDA-induced apoptosis via inhibition of nuclear factor-kappa B (NF-kappa B) activation. These data indicate that LRP may have the potential to act as a neuroprotective agent.
| Item Type: | Article |
|---|---|
| Funders: | Foundation for Subject Construction of Sichuan Academy of Agricultural Sciences [2021XKJS058], High-tech field expansion project of Sichuan Academy of Agricultural Sciences [2018GXTZ-001], Sichuan Association for Science and Technology Young Scholar Project [2018--007], International Communication and Collaboration Project of Sichuan Academy of Agricultural Sciences [2022ZSSFGH09] |
| Uncontrolled Keywords: | Lignosus rhinocerotis; neuroprotective effect; oxidative stress; peptide |
| Subjects: | T Technology > TP Chemical technology |
| Divisions: | Faculty of Pharmacy > Department of Pharmaceutical Life Sciences |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 14 Jun 2024 01:42 |
| Last Modified: | 14 Jun 2024 01:42 |
| URI: | http://eprints.um.edu.my/id/eprint/39448 |
Actions (login required)
 |
View Item |
