Eff, Aprilita Rina Yanti and Huri, Hasniza Zaman and Radji, Maksum and Munim, Abdul and Suyatna, F. D. and Eden, Yonatan (2023) Angiotensin converting enzyme (ACE) inhibitors activity from purified compounds Fructus Phaleria macrocarpa (Scheff) Boerl. BMC Complementary Medicine And Therapies, 23 (1). ISSN 2662-7671, DOI https://doi.org/10.1186/s12906-023-03889-x.
Full text not available from this repository.Abstract
Background Mahkota Dewa Phaleria macrocarpa (Scheff) Boerl.] fruit in vitro and in- vivo can decrease and prevent elevation of the blood pressure, lower plasma glucose levels, possess an antioxidant effect, and recover liver and kidney damage in rats. This study aimed to determine the structure and inhibitory activity of angiotensin-converting enzyme inhibitors (ACE) from the Mahkota Dewa fruit.Methods The fruit powder was macerated using methanol and then partitioned by hexane, ethyl acetate, n-butanol, and water. The fractions were chromatographed on the column chromatography and incorporated with TLC and recrystallization to give pure compounds. The structures of isolated compounds were determined by UV-Visible, FT-IR, MS, proton (H-1-NMR), carbon (C-13-NMR), and 2D-NMR techniques encompassing HMQC and HMBC spectra. The compounds were evaluated for their ACE inhibitory activity, and the strongest compound was determined by the kinetics enzyme inhibition.Results Based on the spectral data, the isolated compounds were determined as 6,4-dihydroxy-4-methoxybenzophenone-2-O-beta-D-glucopyranoside (1), 4,4 ` -dihydroxy-6-methoxybenzophenone-2-O-beta-D-glucopyranoside (2) and mangiferin (3). IC50 values of the isolated compounds 1, 2 and 3 were 0.055, 0.07, and 0.025 mM, respectively.Conclusion The three compounds have ACE inhibitor and mangiferin demonstrated the best ACE inhibitory activity with competitive inhibition on ACE with the type of inhibition kinetics is competitive inhibition.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Education and Research Indonesia and Education fund management agency (LPDP Indonesia) |
| Uncontrolled Keywords: | Phaleria macrocarpa; ACE inhibitor; 6,4-dihydroxy-4-methoxybenzophenone-2-O-beta-D-glucopyranoside; 4,4 ` -dihydroxy-6-methoxybenzophenone-2-O-beta-D-glucopyranoside; Mangiferin |
| Subjects: | R Medicine > RS Pharmacy and materia medica |
| Divisions: | Faculty of Pharmacy > Department of Clinical Pharmacy and Pharmacy Practice |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 07 Jul 2023 03:31 |
| Last Modified: | 07 Jul 2023 03:31 |
| URI: | http://eprints.um.edu.my/id/eprint/38601 |
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