Chua, K.H. and See, K.H. and Thong, Kwai Lin and Puthucheary, S.D. (2011) SpeI restriction enzyme displays greater discriminatory power than XbaI enzyme does in a pulsed-field gel electrophoresis study on 146 clinical burkholderia pseudomallei isolates. Japanese Journal of Infectious Diseases, 64 (3). pp. 228-233. ISSN 1344-6304,
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SpeI_restriction_enzyme_displays_better_discriminatory_power_than_XbaI_enzyme_in_a_PFGE_study_of_146_clinical_isolates_of_Burkholderia.pdf - Published Version Download (747kB) |
Abstract
Restriction enzymes SpeI and XbaI were used in a pulsed-field gel electrophoresis (PFGE) study for molecular characterization of 146 clinical Burkholderia pseudomallei isolates. The PFGE parameters were optimized to enable comparable, reproducible, and robust results. The optimized parameters for both SpeI and XbaI restriction enzymes used in this study were 200 V and a pulse time of 5 to 65 s for a 28-h runtime. Using SpeI, 9 different clusters were identified, whereas 6 clusters were identified by XbaI digestion, which exhibited 85 similarity to SpeI. SpeI (discrimination index D=0.854) showed higher discriminatory power than XbaI did (D=0.464).
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Additional Information: | Corresponding author: Mailing address: Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. Tel: ＋603- 79676607, Fax: ＋603-79676600, E-mail: khchua＠um.edu.my |
| Uncontrolled Keywords: | Molecular Medicine; Restriction enzymes SpeI and XbaI |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms azrahani halim |
| Date Deposited: | 28 Sep 2012 01:55 |
| Last Modified: | 15 Oct 2018 04:21 |
| URI: | http://eprints.um.edu.my/id/eprint/3850 |
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