Lau, Tien-Tien Vicky and Puah, Suat Moi and Tan, Jin-Ai Mary Anne and Merino, Susana and Puthucheary, S. D. and Chua, Kek Heng (2023) Flagellar motility mediates biofilm formation in Aeromonas dhakensis. Microbial Pathogenesis, 177. ISSN 0882-4010, DOI https://doi.org/10.1016/j.micpath.2023.106059.
Full text not available from this repository.Abstract
Aeromonas dhakensis possesses dual flagellar systems for motility under different environments. Flagella -mediated motility is necessary for biofilm formation through an initial attachment of bacteria to the surface, but this has not been elucidated in A. dhakensis. This study investigates the role of polar (flaH, maf1) and lateral (lafB, lafK and lafS) flagellar genes in the biofilm formation of a clinical A. dhakensis strain WT187 isolated from burn wound infection. Five deletion mutants and corresponding complemented strains were constructed using pDM4 and pBAD33 vectors, respectively, and analyzed for motility and biofilm formation using crystal violet staining and real-time impedance-based assays. All mutants were significantly reduced in swimming (p < 0.0001), swarming (p < 0.0001) and biofilm formation using crystal violet assay (p < 0.05). Real-time imped-ance-based analysis revealed WT187 biofilm was formed between 6 to 21 h, consisting of early (6-10 h), middle (11-18 h), and late (19-21 h) stages. The highest cell index of 0.0746 was recorded at 22-23 h and biofilms began to disperse starting from 24 h. Mutants Delta maf1, Delta lafB, Delta lafK and Delta lafS exhibited reduced cell index values at 6-48 h when compared to WT187 which indicates less biofilm formation. Two complemented strains cmaf1 and clafB exhibited full restoration to wild-type level in swimming, swarming, and biofilm formation using crystal violet assay, hence suggesting that both maf1 and lafB genes are involved in biofilm formation through flagella-mediated motility and surface attachment. Our study shows the role of flagella in A. dhakensis biofilm formation warrants further investigations.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Aeromonas dhakensis; Biofilm formation; Flagellar motility; Mutant; Real-time impedance monitoring |
| Subjects: | R Medicine R Medicine > R Medicine (General) > Medical technology |
| Divisions: | Faculty of Medicine Faculty of Medicine > Biomedical Science Department |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 04 Nov 2024 01:39 |
| Last Modified: | 04 Nov 2024 01:39 |
| URI: | http://eprints.um.edu.my/id/eprint/38448 |
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