Investigation of SLC2A1 26177A/G gene polymorphism via high resolution melting curve analysis in Malaysian patients with diabetic retinopathy

Ng, Z.X. and Kuppusamy, U.R. and Tajunisah, I. and Fong, K.C.S. and Chua, K.H. (2012) Investigation of SLC2A1 26177A/G gene polymorphism via high resolution melting curve analysis in Malaysian patients with diabetic retinopathy. Journal of Diabetes and its Complications, 26 (5). pp. 388-392. ISSN 1056-8727,

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Abstract

Purpose In this study, we aimed to investigate the possible association between SLC2A126177A/Gpolymorphism and diabetic retinopathy (DR) in Malaysianpatients with type 2 diabetes. Methods Genomic DNA was extracted from 211 Malaysian type 2 diabeticpatients (100 without retinopathy DNR, 111 with retinopathy) and 165 healthy controls. A highresolutionmelting assay developed in this study was used to detect SLC2A126177A/Gpolymorphism followed by statistical analysis. Results A statistically significant difference in 26177 G minor allele frequency between healthy controls (19.7 %) and total patient group (26.1 %) (p < 0.05, Odd ratio = 1.437, 95% Confidence interval = 1.015�2.035) as well as between healthy controls (19.7 %) and DNR patients (27.5%) (p < 0.05, Odd ratio = 1.546, 95% Confidence interval = 1.024�2.336) was shown in this study. However, when compared between DR and DNR patients, there was no significant difference (p > 0.05). Conclusions This is the first study which shows that SLC2A1 26177G allele is associated with type 2 diabetes in Malaysian population but not with DR.

Item Type: Article
Funders: UNSPECIFIED
Additional Information: Department of Molecular Medicine, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
Uncontrolled Keywords: Diabetic retinopathy; GLUT1; HRM; Polymorphism; SLC2A1
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms azrahani halim
Date Deposited: 02 Nov 2012 01:24
Last Modified: 02 Nov 2012 01:24
URI: http://eprints.um.edu.my/id/eprint/3831

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