Wong, Yin Cheng and Ng, Allan Wee Ren and Chen, Qingwen and Liew, Pei Sheng and Lee, Choon Weng and Sim, Edmund Ui Hang and Narayanan, Kumaran (2023) Phage N15-based vectors for gene cloning and expression in bacteria and mammalian cells. ACS SYNTHETIC BIOLOGY, 12 (4). pp. 909-921. ISSN 2161-5063, DOI https://doi.org/10.1021/acssynbio.2c00580909.
Full text not available from this repository.Abstract
Bacteriophage N15 is the first virus known to deliver linear prophage into Escherichia coli. During its lysogenic cycle, N15 protelomerase (TelN) resolves its telomerase occupancy site (tos) into hairpin telomeres. This protects the N15 prophage from bacterial exonuclease degradation, enabling it to stably replicate as a linear plasmid in E. coli. Interestingly, purely proteinaceous TelN can retain phage DNA linearization and hairpin formation without involving host-or phage-derived intermediates or cofactors in the heterologous environment. This unique feature has led to the advent of synthetic linear DNA vector systems derived from the TelN-tos module for the genetic engineering of bacterial and mammalian cells. This review will focus on the development and advantages of N15-based novel cloning and expression vectors in the bacterial and mammalian environments. To date, N15 is the most widely exploited molecular tool for the development of linear vector systems, especially the production of therapeutically useful miniDNA vectors without a bacterial backbone. Compared to typical circular plasmids, linear N15-based plasmids display remarkable cloning fidelity in propagating unstable repetitive DNA sequences and large genomic fragments. Additionally, TelN-linearized vectors with the relevant origin of replication can replicate extrachromosomally and retain transgenes functionality in bacterial and mammalian cells without compromising host cell viability. Currently, this DNA linearization system has shown robust results in the development of gene delivery vehicles, DNA vaccines and engineering mammalian cells against infectious diseases or cancers, highlighting its multifaceted importance in genetic studies and gene medicine.
| Item Type: | Article |
|---|---|
| Funders: | FRGS grant from the Ministry of Higher Education, Malaysia [FRGS/1/2016/STG05/MUSM/02/2], Monash University Malaysia |
| Uncontrolled Keywords: | bacteriophage N15; protelomerase TelN; telomerase occupancy site; hairpin telomeres; cloning and expression vectors |
| Subjects: | T Technology > TP Chemical technology |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 15 Jun 2024 03:25 |
| Last Modified: | 15 Jun 2024 03:25 |
| URI: | http://eprints.um.edu.my/id/eprint/38278 |
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