Cloning and expression of a Vi mimotope of Salmonella enterica serovar Typhi through nucleotide-nucleotide hybridization approach.l

Chua, Kek Heng and Kee, B. and Thong, Kwai Lin (2009) Cloning and expression of a Vi mimotope of Salmonella enterica serovar Typhi through nucleotide-nucleotide hybridization approach.l. African Journal of Biotechnology, 8 (18). pp. 4623-4626. ISSN 1684-5315,

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Abstract

A recombinant His-Vi protein of Salmonella enterica serovar Typhi was successfully constructed and cloned into an expression vector through a nucleotide-nucleotide hybridization approach. After transformation of the construct into Escherichia coli, the recombinant His-Vi protein with a size of approximately 4 kDa was successfully produced and proven by Western blot analysis. This recombinant protein can be used to detect specific anti-Vi antibody produced by typhoid patients. Overall, the His-Vi recombinant protein could serve as a potential diagnostic reagent to detect S. Typhi infection in an individual.

Item Type:	Article
Funders:	UNSPECIFIED
Additional Information:	Department of Molecular Medicine, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
Uncontrolled Keywords:	Salmonella enterica serovar Typhi, recombinant protein, Vi mimotope.
Subjects:	R Medicine
Divisions:	Faculty of Medicine Faculty of Science > Institute of Biological Sciences
Depositing User:	Ms azrahani halim
Date Deposited:	01 Nov 2012 07:57
Last Modified:	15 Oct 2018 03:26
URI:	http://eprints.um.edu.my/id/eprint/3789

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