Application of genefishing discovery system on differential gene expression study for prokaryotic system

Chua, Kek Heng and Chai, H.C. and Puthucheary, Savithri Devi (2010) Application of genefishing discovery system on differential gene expression study for prokaryotic system. African Journal of Biotechnology, 9 (35). pp. 5629-5631. ISSN 1684-5315,

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Abstract

GeneFishing Discovery is a system applied on disease and gene related research for eukaryotes and the starting material for the study could be either total RNA or mRNA. In this study we demonstrate the use of this discovery system for a prokaryotic system by modifying the eukaryotic protocol using the poly (A)-tailing approach. As an example we compared the gene expression pattern of two closely related species of bacteria under the same family (Burkholderia thailandensis and Burkholderia cepacia) with satisfactory results.

Item Type:	Article
Funders:	UNSPECIFIED
Additional Information:	Corresponding author. E-mail: khchua@um.edu.my. Tel: 603- 79676607. Fax: 603-79676600.
Uncontrolled Keywords:	Genefishing; differential expression; prokaryotic system
Subjects:	R Medicine
Divisions:	Faculty of Medicine
Depositing User:	Ms azrahani halim
Date Deposited:	25 Oct 2012 02:11
Last Modified:	26 Feb 2021 06:53
URI:	http://eprints.um.edu.my/id/eprint/3780

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