2245G/A polymorphism of the receptor for advanced glycation end-products (RAGE) gene is associated with diabetic retinopathy in the Malaysian population

Ng, Zhi Xiang and Kuppusamy, Umah Rani and Tajunisah, Iqbal and Fong, Kenneth Choong Sian and Koay, Adrian Choon Aun and Chua, Kek Heng (2012) 2245G/A polymorphism of the receptor for advanced glycation end-products (RAGE) gene is associated with diabetic retinopathy in the Malaysian population. British Journal of Ophthalmology, 96 (2). pp. 289-292. ISSN 0007-1161, DOI https://doi.org/10.1136/bjophthalmol-2011-300658.

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Abstract

Background/aims The receptor for advanced glycation end-products (RAGE) has been implicated in the pathogenesis of diabetic microvascular complications. The aim of this study was to investigate the association between 2245G/A gene polymorphism of the RAGE gene and retinopathy in Malaysian type 2 diabetic patients. Methods 342 unrelated type 2 diabetic patients (171 with retinopathy (DR), 171 without retinopathy (DNR)) and 235 unrelated healthy subjects from all over Malaysia were recruited for this study. Genomic DNA was isolated from 3�ml samples of whole blood using a modified conventional DNA extraction method. The genotype and allele frequencies of 2245G/A were studied using the polymerase chain reaction�restriction fragment length polymorphism (PCR-RFLP) method. Results A statistically significant difference in 2245A minor allele frequency was found between control (5.5) and DR groups (15.2) (p<0.001, OR=3.06, 95 CI 1.87 to 5.02) as well as between DNR (8.2) and DR (15.2) groups (p<0.01, OR=2.01, 95 CI 1.24 to 3.27). However, when the frequency was compared between control and DNR groups, there was no significant difference (p>0.05). Conclusions This is the first study that shows an association between the 2245A allele of the RAGE gene and development of diabetic retinopathy in the Malaysian population.

Item Type:	Article
Funders:	UNSPECIFIED
Additional Information:	Department of Molecular Medicine, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA ---> Correspondence to Professor Kek Heng Chua, Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia; khchua@um.edu.my
Uncontrolled Keywords:	Molecular Medicine
Subjects:	R Medicine
Divisions:	Faculty of Medicine
Depositing User:	Ms azrahani halim
Date Deposited:	25 Oct 2012 02:06
Last Modified:	02 Aug 2019 05:57
URI:	http://eprints.um.edu.my/id/eprint/3779

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