The behaviour of selected oral bacteria in single- and mixed-species biofilms

Wan Nordini Hasnor, W.I. and Razak, Fathilah Abdul and Yusoff, Mohd Md and Rahim, Zubaidah Haji Abdul (2008) The behaviour of selected oral bacteria in single- and mixed-species biofilms. Journal of Biological Sciences, 8 (6). pp. 1001-1007. ISSN 1727-3048, DOI https://doi.org/10.3923/jbs.2008.1001.1007.

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Abstract

The objective of this study was to investigate and compare the adhering capacity of early colonizers of oral biofilm in a dynamic environment. The study was carried out by inoculating the early colonisers of oral biofilm (Streptococcus mitis, Streptococcus sanguinis and Actinomyces sp.) singly and in a mixture to the experimental pellicle in an artificial mouth (NAM) model for 24 h. This will form the respective 24 h single- and mixed-species biofilm. The bacterial population adhering to the experimental pellicle was determined and expressed as colony forming unit per ml (cfu mL-1). The adhered bacterial population was further confirmed using the Scanning Electron Microscope. It was found that S. mitis demonstrated maximum adherence (1153.33±132.46×104 cfu mL-1), followed by S. sanguinis (183.00±10.33×104 cfu mL-1) and by Actinomyces sp. (42.33±3.20×104 cfu mL-1). The adhering capacity of bacteria when present in a mixture of two-species was found to be reduced. However in a mixture of three species, it was found that the bacterial adherence was slightly increased. The difference in the adherence capacity of these bacteria to the experimental pellicle was found to be statistically significant (p<0.05). The results obtained in this study suggest that selected oral bacteria behave differently in a single- and mixed species biofilms. © 2008 Asian Network for Scientific Information.

Item Type:	Article
Funders:	UNSPECIFIED
Additional Information:	Export Date: 10 September 2012 Source: Scopus Language of Original Document: English Correspondence Address: Rahim, Z.H.A.; Department of Oral Biology, Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia References: Black, C., Allan, I., Ford, S.K., Wilson, M., McNab, R., Biofilm-specific surface properties and protein expression in oral Streptococcus sanguis (2004) Arch. Oral Biol, 49, pp. 295-304; Bleiweis, A.S., Oyston, P.C.F., Adhesion and Cohesion of Plaque Microflora: A Function of Microbial Fimbriae and Fibrils (1993) Caniology for the Nineties, pp. 287-285. , Bowen W.H. and A.L. Tabak Eds, University of Rochester Press, New York, pp; Branda, S.S., Vik, A., Friedman, L., Kolter, R., Biofilms: The matrix revistod (2005) Trends Microbiol, 13, pp. 20-26; Carlén, A., Olsson, J., Ramberg, P., Saliva mediated adherence, aggregation and prevalence in dental plaque of Streptococcus mutans, Streptococcus sanguis and Actinomyces sp. in young and elderly humans (1996) Arch. Oral Biol, 41, pp. 1133-1140; Ceri, H., Olson, M.E., Stremick, C., Read, R.R., Morck, D., The Calgary biofilm device: New technology for rapid determination of antibiotic susceptibilites of bacterial biofilms (1999) J. Clin. Microbiol, 37, pp. 1771-1776; Christensen, B.B., Haagensen, J.A.S., Heydon, A., Molin, S., Metabolic commensalism and competition in a 2-species microbial consortium (2002) Applied Environ. Microbiol, 68, pp. 2495-2502; Costerton, J.W., Discussion: Introduction to biofilm (1999) Int. J. Antimicrob. Agents, 11, pp. 217-221; Fathilah, A.R., Y. Othman and Z.H.A. Rahim, 1999. An in vitro study on the anti-adherence properties of mouthrinses containing chlorhexidene gluconate and hexitidine. Am. Dentistry, 6:17-20Fathilah, A.R., Rahim, Z.H.A., The anti-adherence effect of Piper betle and Psidium guajava extracts on the adhesion of early settlers in dental plaque to saliva-coated glass surfaces (2003) J. Oral. Sci, 45, pp. 206-210; Jefferson, K.K., Mini review: What drives bacteria to produce a biofilm (2004) FEMS Microbiol. Lett, 235, pp. 163-173; Kreth, J., Merritt, J., Sni, W., Qi, F., Competition and coexistence between Streptococcus mutans and Streptococcus sanguinis in the dental biofilm (2005) J. Bacteriol, 187, pp. 7193-7203; Lagacé, L., Jacques, M., Mafu, A.A., Roy, D., Composition of maple sap microflora and collection system biofilms evaluated by Scanning Electron Microscopy and denaturamig gradient gel electrophoresis (2006) Int. J. Food Microbiol, 109, pp. 9-18; Li, J., Helmerhorst, E.J., Leone, C.W., Troxler, R.F., Yaskell, T., Identification of early microbial colonizers in human dental biofilm (2004) J. Applied Microbiol, 97, pp. 1311-1318; Listgarten, M.A., 1999. Formation of Dental Plaque and Other Oral Biofilms. In: Dental Plaque Revisited, Oral Blofilms in Health and Disease, Newman, H.N. and M. Wilson (Eds.). BioLine, United Kingdom, pp: 187-209Mah, T.F.C., O'Toole, G.A., Mechanisms of biofilm resistance to antimicrobial agents (2001) Trends Microbiol, 9, pp. 34-39; Maiden, M.F.J., Lai, C.H., Tanner, A., Characteristics of Oral Gram-Positive Species (1992) Contemporary Oral Microbiology and Immunology, pp. 342-372. , Slots, J. and M.A. Taubman Eds, Mosby Year Book, St Louis, pp; Marsh, P.D., Martin, M.V., (1999) Oral Microbiology, , 4th Edn, Wright Great Britain; Palmer, R.J.J., Gordon, S.M., Cisar, T.O., Kolenbrander, P.E., Coaggregation-mediated interactions of Streptococci and Actinomyces detected in initial human dental plaque (2003) J. Bacteriol, 185, pp. 3400-3409; Rahim, Z.H.A., Fathilah, A.R., Irwan, S., Hasnor, W.I.W.N., An artificial mouth system (NAM model) for oral biofilm research (2008) Res. J. Microbiol, 3, pp. 466-473; Singh, R., Paul, D., Jain, R.K., Biofilms: Implication in bioremediation (2006) Trends Microbiol, 14, pp. 389-397; Slots, J., Taubman, M.A., (1992) Contemporary Oral Microbiology and Immunology, , 1st Edn, Mosby Year Book, Mosby; Tait, K., Sutherland, I.W., Antagonistic interactions amongst bacteriocin-producing enteric bacteria in dual species biofilm (2002) J. Applied Microbiol, 93, pp. 345-352; Watnick, P., Kolter, R., Biofilm, city of microbes (2000) J. Bacteriol, 182, pp. 2675-2679
Uncontrolled Keywords:	Bacterial adherence; Dynamic environment; Early colonizers; NAM model; Planktonic environment
Subjects:	R Medicine > RK Dentistry
Divisions:	Faculty of Dentistry > Dept of Oral Biology
Depositing User:	Mr Ahmad Azwan Azman
Date Deposited:	10 Oct 2012 02:11
Last Modified:	17 Dec 2020 03:56
URI:	http://eprints.um.edu.my/id/eprint/3755

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