Ji, Huihui and Yi, Quanyong and Chen, Lishuang and Wong, Liping and Liu, Yanfen and Xu, Guodong and Zhao, Jun and Huang, Tianyi and Li, Bin and Yang, Yong and Li, Wenxia and Han, Liyuan and Duan, Shiwei (2020) Circulating miR-3197 and miR-2116-5p as novel biomarkers for diabetic retinopathy. Clinica Chimica Acta, 501. pp. 147-153. ISSN 0009-8981, DOI https://doi.org/10.1016/j.cca.2019.10.036.
Full text not available from this repository.Abstract
Diabetic retinopathy (DR) is the leading cause of vision loss among older adults. The goal of this case-control study was to identify circulating miRNAs for the diagnosis of DR. The miRNeasy Serum/Plasma Kit was used to extract serum miRNAs. The mu Paraflo (TM) MicroRNA microarray was used to detect the expression levels of the miRNAs. The miRWalk algorithm was applied to predict the target genes of the miRNAs, which were further confirmed by the dual luciferase reporter gene system in HEK293T cells. A microarray was performed between 5 DR cases and 5 age-, sex-, body mass index-, and duration of diabetes-matched type 2 diabetic (T2DM) controls. The quantitative reverse transcription polymerase chain reaction technique was used to validate the differentially expressed circulating miRNAs in 45 DR cases and 45 well-matched controls. Receiver operating characteristic (ROC) curve analysis was used to evaluate the performance of the circulating miRNAs as diagnostic biomarkers for DR. Our microarray analysis screened out miR-2116-5p and miR-3197 as significantly up regulated in DR cases compared with the controls. Furthermore, two miRNAs were validated in the 45 DR cases and 45 controls. The ROC analysis suggested that both miR-3197 and miR-2116-5p distinguished DR cases from controls. An additional dual-luciferase reporter gene assay confirmed that notch homolog 2 (NOTCH2) was the target gene of miR-2116-5p. Both miR-3197 and miR-2116-5p were identified as promising diagnostic biomarkers for DR. Future research is still needed to explore the molecular mechanisms of miR-3197 and miR-2116-5p in the pathogenesis of DR.
| Item Type: | Article |
|---|---|
| Funders: | National Key R&D Program of China [Grant No: 2017YFC1310902, 2018YFC1315305], Ningbo Health Branding Subject Fund [Grant No: PPXK2018-02], Natural Science Foundation of Zhejiang Province [Grant No: LY17H260002], Sanming Project of Medicine in Shenzhen [Grant No: SZSM201803080] |
| Uncontrolled Keywords: | Diabetic retinopathy; Circulating miRNAs; miR-3197; miR-2116-5p; NCOR2; NOTCH2 |
| Subjects: | R Medicine > R Medicine (General) R Medicine > RA Public aspects of medicine |
| Divisions: | Faculty of Medicine > Social & Preventive Medicine |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 07 Nov 2024 00:52 |
| Last Modified: | 07 Nov 2024 00:52 |
| URI: | http://eprints.um.edu.my/id/eprint/36921 |
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