Khawory, Muhammad Hidhir and Sain, Amyra Amat and Rosli, Mohamad Afiq Aizuddin and Ishak, Muhammad Syafiq and Noordin, Mohamed Ibrahim and Wahab, Habibah A. (2020) Effects of gamma radiation treatment on three different medicinal plants: Microbial limit test, total phenolic content, in vitro cytotoxicity effect and antioxidant assay. Applied Radiation and Isotopes, 157. ISSN 0969-8043, DOI https://doi.org/10.1016/j.apradiso.2019.109013.
Full text not available from this repository.Abstract
The aim of this study is to evaluate the effects of gamma radiation treatment on three medicinal plants, namely Euodia malayana, Gnetwn gnemon and Khaya senegalensis at two different forms; methanol leaf extracts and dried leaves respectively. Experimental procedure: The microbial limit test (MLT) studies indicated the suitable dosage of minimum and maximum gamma irradiation for leaf extracts as well as dried leaves of all the tested medicinal plants. Quantitative analysis of total phenolic content (TPC) analysis is based on calorimetric measurements determined using the Folin-Ciocalteu reagent with gallic acid (GA) used as the reference. In vitro cytotoxicity assay by using fibroblast (L929) cell lines was performed on each plant to determine the toxicity effect which sodium dodecyl sulfate (SDS) as the positive control. DPPH (2,2-diphenyl-1-picryl-hydrazyl) assay was conducted by using vitamin C and GA as the positive controls to determine the antioxidant property of each plant. Results and conclusion: The MLT analysis indicated that the suitable dosage gamma irradiation for leaf extracts was 6-12 kGy and dried leaves were 9-13 kGy. The amount of GA concentration in each plant increased significantly from 30-51 mg GAE g(-1) before treatment to 57-103 mg GAE g(-1) after treatment with gamma radiation. This showed no significant effect of in vitro cytotoxicity activity before and after treatment with gamma irradiation in this study. Effective concentration (EC50) values of Khaya senegalensis plant reduced significantly (P <= 0.005) from 44.510 mu g/ml before treatment to 24.691 mu g/ml after treatment with gamma radiation, which indicate an increase of free radical scavenging activity.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Energy, Science, Technology, Environmental and Climate Change (MESTECC), undamental Research Grant Scheme [FP0813B023] |
| Additional Information: | 16th Latin American Conference of Analysis by X-Ray Techniques (SARX) / 6th Conference of Medical Physics in the Frontier (JFMF), Pucon, PEOPLES R CHINA, NOV 04-07, 2018 |
| Uncontrolled Keywords: | Medicinal plants; Microbial limit test; Total phenolic content; In vitro cytotoxicity; DPPH (2 2-Diphenyl-1-picryl-hydrazyl) |
| Subjects: | Q Science > QK Botany R Medicine |
| Divisions: | Faculty of Science |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 05 Oct 2023 03:13 |
| Last Modified: | 05 Oct 2023 03:13 |
| URI: | http://eprints.um.edu.my/id/eprint/36846 |
Actions (login required)
 |
View Item |
