Hooi, Y. T. and Ong, K. C. and Tan, S. H. and Perera, D. and Wong, K. T. (2020) Coxsackievirus A16 in a 1-Day-Old mouse model of central nervous system infection shows lower neurovirulence than enterovirus A71. Journal of Comparative Pathology, 176. pp. 19-32. ISSN 00219975, DOI https://doi.org/10.1016/j.jcpa.2020.02.001.
Full text not available from this repository.Abstract
Coxsackievirus A16 (CV-A16) and enterovirus A71 (EV-A71) are the major causes of hand, foot and mouth disease in young children. Although less so with CV-A16, both viruses are associated with serious neurological syndromes, but the differences between their central nervous system infections remain unclear. We conducted a comparative infection study using clinically-isolated CV-A16 and EV-A71 strains in a 1-day-old mouse model to better understand the neuropathology and neurovirulence of the viruses. New serotype-specific probes for in situ hybridization were developed and validated to detect CV-A16 and EV-A71 RNA in infected tissues. Demonstration of CV-A16 virus antigens/RNA, mainly in the brainstem and spinal cord neurons, confirmed neurovirulence, but showed lower densities than in EV-A71 infected animals. A higher lethal dose(50) for CV-A16 suggested that CV-A16 is less neurovirulent. Focal virus antigens/RNA in the anterior horn white matter and adjacent efferent motor nerves suggested that neuroinvasion is possibly via retrograde axonal transport in peripheral motor nerves. (C) 2020 Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Higher Education , Malaysia Government [Grant No: PG159-2015A], Universiti Malaya |
| Uncontrolled Keywords: | Coxsackievirus A16; Enterovirus A71; Mouse model; Neurovirulence |
| Subjects: | R Medicine > R Medicine (General) R Medicine > RC Internal medicine |
| Divisions: | Faculty of Medicine > Biomedical Science Department Faculty of Medicine > Pathology Department |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 06 Nov 2024 01:24 |
| Last Modified: | 06 Nov 2024 01:24 |
| URI: | http://eprints.um.edu.my/id/eprint/36782 |
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