Lai, Meng Yee and Ooi, Choo Huck and Jaimin, Joel Judson and Lau, Yee Ling (2020) Evaluation of WarmStart Colorimetric Loop-Mediated Isothermal Amplification Assay for Diagnosis of Malaria. American Journal of Tropical Medicine and Hygiene, 102 (6). pp. 1370-1372. ISSN 0002-9637, DOI https://doi.org/10.4269/ajtmh.20-0001.
Full text not available from this repository.Abstract
The incidence of zoonotic malaria, Plasmodium knowlesi, infection is increasing and now is the major cause of malaria in Malaysia. Here, we describe a WarmStart colorimetric loop-mediated isothermal amplification (LAMP) assay for the detection of Plasmodium spp. The detection limit for this assay was 10 copies/mu L for P knowlesi and Plasmodium ovale and 1 copy/mu L for Plasmodium falciparum, Plasmodium vivax, and Plasmodium malariae. To test clinical sensitivity and specificity, 100 microscopy-positive and 20 malaria-negative samples were used. The WarmStart colorimetric LAMP was 98% sensitive and 100% specific. Amplification products were visible for direct observation, thereby eliminating the need for post-amplification processing steps. Therefore, WarmStart colorimetric LAMP is suitable for use in resource-limited settings.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Borneo; Colorimetry; Humans; Malaria; Nucleic Acid Amplification techniques; Plasmodium knowlesi; Sensitivity and specificity; Species specificity |
| Subjects: | R Medicine R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine |
| Divisions: | Faculty of Medicine Faculty of Medicine > Parasitology Deparment |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 31 Dec 2023 04:05 |
| Last Modified: | 31 Dec 2023 04:05 |
| URI: | http://eprints.um.edu.my/id/eprint/36657 |
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