Teoh, Boon-Teong and Chin, Kim-Ling and Samsudin, Nur-Izyan and Loong, Shih-Keng and Sam, Sing-Sin and Tan, Kim-Kee and Khor, Chee-Sieng and Abd-Jamil, Juraina and Zainal, Nurhafiza and Wilder-Smith, Annelies and Zandi, Keivan and Abu Bakar, Sazaly (2020) A reverse transcription loop-mediated isothermal amplification for broad coverage detection of Asian and African Zika virus lineages. BMC Infectious Diseases, 20 (1). ISSN 1471-2334, DOI https://doi.org/10.1186/s12879-020-05585-4.
Full text not available from this repository.Abstract
BackgroundEarly detection of Zika virus (ZIKV) infection during the viremia and viruria facilitates proper patient management and mosquito control measurement to prevent disease spread. Therefore, a cost-effective nucleic acid detection method for the diagnosis of ZIKV infection, especially in resource-deficient settings, is highly required.MethodsIn the present study, a single-tube reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the detection of both the Asian and African-lineage ZIKV. The detection limit, strain coverage and cross-reactivity of the ZIKV RT-LAMP assay was evaluated. The sensitivity and specificity of the RT-LAMP were also evaluated using a total of 24 simulated clinical samples. The ZIKV quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assay was used as the reference assay.ResultsThe detection limit of the RT-LAMP assay was 3.73 ZIKV RNA copies (probit analysis, P <= 0.05). The RT-LAMP assay detected the ZIKV genomes of both the Asian and African lineages without cross-reacting with other arthropod-borne viruses. The sensitivity and specificity of the RT-LAMP assay were 90% (95% CI=59.6-98.2) and 100% (95% CI=78.5-100.0), respectively. The RT-LAMP assay detected ZIKV genome in 9 of 24 (37.5%) of the simulated clinical samples compared to 10 of 24 (41.7%) by qRT-PCR assay with a high level of concordance (kappa =0.913, P<0.001).ConclusionThe RT-LAMP assay is applicable for the broad coverage detection of both the Asian and African ZIKV strains in resource-deficient settings.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Infectious disease; Diagnostics; RT-LAMP; ZIKV; Mosquito; Vector; Vector-borne |
| Subjects: | R Medicine R Medicine > RA Public aspects of medicine R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 29 Nov 2023 01:27 |
| Last Modified: | 29 Nov 2023 04:34 |
| URI: | http://eprints.um.edu.my/id/eprint/36186 |
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