Tang, S.W. and AbuBakar, Sazaly and Devi, S. and Puthucheary, S. and Pang, T. (1997) Induction and characterization of heat shock proteins of Salmonella typhi and their reactivity with sera from patients with typhoid fever. Infection and immunity, 65 (7). pp. 2983-6. ISSN 0019-9567, DOI 9199477.
Full text not available from this repository.Abstract
The heat shock protein (HSP) response of Salmonella typhi following exposure to elevated growth temperatures was studied. Three major proteins with molecular sizes of 58, 68, and 88 kDa were abundantly expressed when S. typhi cells were shifted from 37 to 45 degrees C and to 55 degrees C. These proteins were also constitutively expressed at 37 degrees C. Western blotting and immunoprecipitation studies with anti-HSP monoclonal antibodies revealed that the 58- and 68-kDa proteins were analogous to the GroEL and DnaK proteins, respectively, of Escherichia coli. These HSPs are also abundantly present in the outer membrane fraction of disrupted cells and, to a lesser extent, in the cytosol. Immunoblotting experiments with sera from patients with a culture-positive diagnosis of typhoid fever showed the presence of antibodies to these HSPs. Nine of twelve sera reacted with the 58-, 68-, and 88-kDa proteins, while three sera reacted only with the 68- and 88-kDa proteins. All 10 sera from healthy individuals showed no binding to these HSPs. In light of the well-documented roles of HSPs in the pathogenesis of microbial infections and as immunodominant antigens, these findings may be relevant for a better understanding of disease processes and for the future development of diagnostic and preventive strategies.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Additional Information: | Institute of Postgraduate Studies & Research, University of Malaya |
| Uncontrolled Keywords: | Antibodies, Monoclonal; Bacterial Proteins/immunology; Blotting, Western; |
| Subjects: | R Medicine > R Medicine (General) |
| Divisions: | Faculty of Medicine |
| Depositing User: | Mr. Faizal Hamzah |
| Date Deposited: | 04 Mar 2011 04:35 |
| Last Modified: | 07 Feb 2019 07:27 |
| URI: | http://eprints.um.edu.my/id/eprint/356 |
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