Zulkefli, Noorfatin Jihan and Teh, Cindy Shuan Ju and Mariappan, Vanitha and Ngoi, Soo Tein and Vadivelu, Jamunarani S. and Ponnampalavanar, Sasheela Sri La Sri and Chai, Lay Ching and Chong, Chun Wie and Yap, Ivan Kok Seng and Vellasamy, Kumutha Malar (2021) Genomic comparison and phenotypic profiling of small colony variants of Burkholderia pseudomallei. PLoS ONE, 16 (12). ISSN 1932-6203, DOI https://doi.org/10.1371/journal.pone.0261382.
Full text not available from this repository.Abstract
Burkholderia pseudomallei (B. pseudomallei) is an intracellular pathogen that causes melioidosis, a life-threatening infection in humans. The bacterium is able to form small colony variants (SCVs) as part of the adaptive features in response to environmental stress. In this study, we characterize the genomic characteristics, antimicrobial resistance (AMR), and metabolic phenotypes of B. pseudomallei SCV and wild type (WT) strains. Whole-genome sequence analysis was performed to characterize the genomic features of two SCVs (CS and OS) and their respective parental WT strains (CB and OB). Phylogenetic relationship between the four draft genomes in this study and 19 publicly available genomes from various countries was determined. The four draft genomes showed a close phylogenetic relationship with other genomes from Southeast Asia. Broth microdilution and phenotype microarray were conducted to determine the AMR profiles and metabolic features (carbon utilization, osmolytes sensitivity, and pH conditions) of all strains. The SCV strains exhibited identical AMR phenotype with their parental WT strains. A limited number of AMR-conferring genes were identified in the B. pseudomallei genomes. The SCVs and their respective parental WT strains generally shared similar carbon-utilization profiles, except for D,L-carnitine (CS), g-hydroxybutyric acid (OS), and succinamic acid (OS) which were utilized by the SCVs only. No difference was observed in the osmolytes sensitivity of all strains. In comparison, WT strains were more resistant to alkaline condition, while SCVs showed variable growth responses at higher acidity. Overall, the genomes of the colony morphology variants of B. pseudomallei were largely identical, and the phenotypic variations observed among the different morphotypes were strain-specific.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Higher Education, Malaysia under the Fundamental Research Grant Scheme (FRGS)[FRGS/1/2020/SKK0/UM/02/7 (FP018-2020)], Universiti Malaya Research Grant (UMRG)[RP013B-13HTM] |
| Uncontrolled Keywords: | Staphylococcus-aureus;Melioidosis;Expression;Resistance;Bacteria;Sequence |
| Subjects: | Q Science > QR Microbiology R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 18 Oct 2022 03:50 |
| Last Modified: | 18 Oct 2022 03:50 |
| URI: | http://eprints.um.edu.my/id/eprint/35270 |
Actions (login required)
 |
View Item |
