Madkhali, Aymen M. and Abdulhaq, Ahmed A. and Atroosh, Wahib M. and Ghzwani, Ahmad Hassn and Zain, Khalid Ammash and Ghailan, Khalid Y. and Hamali, Hassan A. and Mobarki, Abdullah A. and Eisa, Zaki M. and Lau, Yee-Ling and Al-Mekhlafi, Hesham M. (2021) The return of chloroquine-sensitive Plasmodium falciparum parasites in Jazan region, southwestern Saudi Arabia over a decade after the adoption of artemisinin-based combination therapy: Analysis of genetic mutations in the pfcrt gene. Parasitology Research, 120 (11). pp. 3771-3781. ISSN 0932-0113, DOI https://doi.org/10.1007/s00436-021-07323-4.
Full text not available from this repository.Abstract
This study investigated the polymorphism in the P. falciparum chloroquine resistance transporter (pfcrt) gene 11 years after chloroquine (CQ) cessation in Jazan region, southwestern Saudi Arabia. Two hundred and thirty-five P. falciparum isolates were amplified to detect mutations in the pfcrt gene. The pfcrt 76 T molecular marker for CQ resistance was detected in 66.4% (156/235) of the isolates, while the K76 CQ-sensitive wild type was detected in 33.6%. The pfcrt 74I and pfcrt 75E point mutations were each found to be present in 56.2% of isolates, while only four isolates (1.7%) were found to carry the pfcrt 72S mutation. Moreover, four pfcrt haplotypes were identified as follows: the CVIET triple-allele (56.2%), SVMET double-allele (1.7%) and CVMNT single-allele (8.5%) mutant haplotypes and the CVMNK wild haplotype (33.6%). The analysis also revealed significant associations between the prevalence of mutant pfcrt alleles and haplotypes and the age group, governorate and nationality of the patients as well as the parasitaemia level (p < 0.05). The findings provide evidence of the potential re-emergence of CQ-susceptible P. falciparum strains in Jazan region over a decade after CQ discontinuation, with about one third of the isolates analysed carrying the pfcrt K76 CQ-sensitive wild allele and the CVMNK ancestral wild haplotype. Although the reintroduction of CQ cannot be recommended at present in Saudi Arabia, these findings support the rationale for a potential future role for CQ in malaria treatment. Therefore, continuous molecular and in vitro monitoring mutations of pfcrt polymorphism in Jazan region is highly recommended.
| Item Type: | Article |
|---|---|
| Funders: | Deanship of Scientific Research, Jazan University (Vector-Borne Diseases Research Group)[RG-2-1] |
| Uncontrolled Keywords: | Chloroquine;Drug resistance;Infectious diseases;Malaria; Pfcrt;Plasmodium falciparum;Saudi Arabia |
| Subjects: | Q Science > QR Microbiology R Medicine R Medicine > RA Public aspects of medicine R Medicine > RB Pathology |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 14 Sep 2022 01:26 |
| Last Modified: | 14 Sep 2022 01:26 |
| URI: | http://eprints.um.edu.my/id/eprint/34447 |
Actions (login required)
 |
View Item |
