Mohamed, E. and Abdul-Rahman, P.S. and Doustjaialil, S.R. and Chen, Y. and Lim, B.K. and Omar, S.Z. and Bustam, A.Z. and Singh, V.A. and Mohd-Taib, N. and Yip, C.H. and Hashim, Onn Haji (2008) Lectin-based electrophoretic analysis of the expression of the 35 kDa inter-alpha-trypsin inhibitor heavy chain H4 fragment in sera of patients with five different malignancies. Electrophoresis, 29 (12). pp. 2645-2650. ISSN 0173-0835, DOI https://doi.org/10.1002/elps.200700828.
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Abstract
A 35 kDa glycoprotein whose abundance was previously demonstrated to be enhanced in sera of patients with endometrial adenocarcinoma (n = 12), was isolated from pooled sera of three of the cancer patients using champedak galactose-binding lectin affinity chromatography in the present study. Subjecting it to 2-DE and MS/MS, the glycoprotein was identified as the O-glycosylated fragment of inter-a-trypsin inhibitor heavy chain H4 (ITIH4). When compared to control sera (n = 17), expression of the 35 kDa ITIH4 cleavage fragment was demonstrated to be significantly enhanced in sera of patients with breast carcinoma (n = 10), epithelial ovarian carcinoma (n = 10), and germ cell ovarian carcinoma (n = 10) but not in patients with nasopharyngeal carcinoma (n = 13) and osteosarcoma (n = 7). The lectin-based electrophoretic bioanalytical method adopted in the present study may be used to assess the physiological relevance of ITIH4 fragmentation and its correlation with different malignancies, their stages and progression.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Additional Information: | Correspondence: Professor Onn Haji Hashim, Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia E-mail: onnhashim@um.edu.my Fax: 1603-79474957 |
| Uncontrolled Keywords: | Cancer; Champedak; Inter-a-trypsin inhibitor heavy chain H4; Lectin; Serum |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms Haslinda Lahuddin |
| Date Deposited: | 10 Jul 2012 01:23 |
| Last Modified: | 24 Oct 2019 08:22 |
| URI: | http://eprints.um.edu.my/id/eprint/3436 |
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