Islam, Rafiqul and Toida, Yu and Chen, Fei and Tanaka, Toru and Inoue, Satoshi and Kitamura, Tetsuya and Yoshida, Yasuhiro and Chowdhury, Abu Faem Mohammad Almas and Aly Ahmed, Hany Mohamed and Sano, Hidehiko (2021) Histological evaluation of a novel phosphorylated pullulan-based pulp capping material: An in vivo study on rat molars. International Endodontic Journal, 54 (10). pp. 1902-1914. ISSN 0143-2885, DOI https://doi.org/10.1111/iej.13587.
Full text not available from this repository.Abstract
Aim To evaluate the dental pulp response to a novel mineral trioxide aggregate containing phosphorylated pullulan (MTAPPL) in rats after direct pulp capping. Methods Ninety-six cavities were prepared in the maxillary first molars of 56 male Wistar rats. The dental pulps were intentionally exposed and randomly divided into four groups according to the application of pulp capping materials: MTAPPL; phosphorylated pullulan (PPL); a conventional MTA (Nex-Cem MTA, NCMTA; positive control); and Super-Bond (SB; negative control). All cavities were restored with SB and observed for pulpal responses at 1-, 3-, 7- and 28-day intervals using a histological scoring system. Statistical analysis was performed using Kruskal-Wallis and Mann-Whitney U-test with Bonferroni's correction, and the level of significance was set at 0.05. DMP1 and CD34 antigen were used to evaluate odontoblast differentiation and pulpal vascularization, respectively. Results On day 1, mild inflammatory cells were present in MTAPPL and NCMTA groups; fewer inflammatory cells were present in the PPL, whereas SB was associated with a mild-to-moderate inflammatory response. A significant difference was observed between PPL and SB (p < .05). No mineralized tissue deposition was observed. On day 3, moderate-to-severe inflammatory cells were present in PPL and SB, whereas MTAPPL and NCMTA had a mild inflammatory response. Initial mineralized tissue deposition was observed in the NCMTA, MTAPPL and SB. A significant difference was observed between MTAPPL and PPL (p < .05). On day 7, a thin layer of mineralized tissue was observed in all tested groups with no or mild inflammatory response. On day 28, no inflammatory response was observed in MTAPPL, whereas NCMTA, PPL and SB had mild inflammatory responses. A significant difference was observed between MTAPPL and SB (p < .05). Complete mineralized tissue barrier formation was observed in MTAPPL, NCMTA and PPL with no significant difference (p > .05). SB exhibited incomplete mineralized tissue barriers, significantly different from NCMTA, MTAPPL and PPL (p < .05). The staining with CD34 was positive in all the groups on all observation days. Conclusion The favourable pulpal responses and induction of mineralized tissue formation associated with MTAPPL indicate its potential application as a direct pulp capping material.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT) [17K11695], Ministry of Education, Science and Culture of Japan [17K11696], Ministry of Education, Science and Culture of Japan [19K10123], Ministry of Education, Science and Culture of Japan [19K24110] [20K09950] |
| Uncontrolled Keywords: | Direct pulp capping; Mineral trioxide aggregate; Phosphorylated pullulan; Pulp capping material |
| Subjects: | R Medicine > RK Dentistry R Medicine > RK Dentistry > Oral surgery |
| Divisions: | Faculty of Dentistry |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 09 Jun 2022 06:58 |
| Last Modified: | 09 Jun 2022 06:58 |
| URI: | http://eprints.um.edu.my/id/eprint/34352 |
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