Oka, Yuichiro and Doi, Miyuki and Taniguchi, Manabu and Tiong, Sheena Yin Xin and Akiyama, Hisanori and Yamamoto, Takuto and Iguchi, Tokuichi and Sato, Makoto (2021) Interstitial axon collaterals of callosal neurons form association projections from the primary somatosensory to motor cortex in mice. Cerebral Cortex, 31 (11). pp. 5225-5238. ISSN 1047-3211, DOI https://doi.org/10.1093/cercor/bhab153.
Full text not available from this repository.Abstract
Association projections from cortical pyramidal neurons connect disparate intrahemispheric cortical areas, which are implicated in higher cortical functions. The underlying developmental processes of these association projections, especially the initial phase before reaching the target areas, remain unknown. To visualize developing axons of individual neurons with association projections in the mouse neocortex, we devised a sparse labeling method that combined in utero electroporation and confocal imaging of flattened and optically cleared cortices. Using the promoter of an established callosal neuron marker gene that was expressed in over 80% of L2/3 neurons in the primary somatosensory cortex (S1) that project to the primary motor cortex (M1), we found that an association projection of a single neuron was the longest among the interstitial collaterals that branched out in L5 from the earlier-extended callosal projection. Collaterals to M1 elongated primarily within the cortical gray matter with little branching before reaching the target. Our results suggest that dual-projection neurons in S1 make a significant fraction of the association projections to M1, supporting the directed guidance mechanism in long-range corticocortical circuit formation over random projections followed by specific pruning.
| Item Type: | Article |
|---|---|
| Funders: | Takeda Science Foundation (TSF), Naito Memorial Foundation, Japanese Society for Promoting Science (JPSP KAKENHI) [JP23800027] |
| Uncontrolled Keywords: | Barrel cortex; Corticocortical projection; Inter-areal connection; Optical tissue clearing; Sensorimotor integration |
| Subjects: | R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 08 Jul 2022 04:16 |
| Last Modified: | 08 Jul 2022 04:16 |
| URI: | http://eprints.um.edu.my/id/eprint/33984 |
Actions (login required)
 |
View Item |
