Inhibition of quorum sensing-controlled virulence factor production in pseudomonas aeruginosa PAO1 by Ayurveda Spice Clove (Syzygium Aromaticum) bud extract

Krishnan, T. and Yin, W.F. and Chan, Kok Gan (2012) Inhibition of quorum sensing-controlled virulence factor production in pseudomonas aeruginosa PAO1 by Ayurveda Spice Clove (Syzygium Aromaticum) bud extract. Sensors, 12 (4). pp. 4016-4030. ISSN 1424-8220, DOI https://doi.org/10.3390/s120404016.

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Official URL: https://doi.org/10.3390/s120404016

Abstract

Quorum sensing controls the virulence determinants in most proteobacteria. In this work, the hexane, chloroform and methanol extracts of an Ayurveda spice, namely clove (Syzygium aromaticum), shown anti-quorum sensing activity. Hexane and methanol extracts of clove inhibited the response of C. violaceum CV026 to exogenously supplied N-hexanoylhomoserine lactone, in turn preventing violacein production. Chloroform and methanol extracts of clove significantly reduced bioluminescence production by E. coli [pSB1075] grown in the presence of N-(3-oxododecanoyl)-l-homoserine lactone. We demonstrated that clove extract inhibited quorum sensing-regulated phenotypes in Pseudomonas aeruginosa PA01, including expression of lecA::lux (by hexane extract), swarming (maximum inhibition by methanol extract), pyocyanin (maximum inhibition by hexane extract). This study shows that the presence of natural compounds that exhibit anti-quorum sensing activity in the clove extracts may be useful as the lead of anti-infective drugs.

Item Type: Article
Funders: UNSPECIFIED
Additional Information: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia; E-Mails: thibavengdes@yahoo.com (T.K.); yinwaifong@yahoo.com (W.F.Y.).
Uncontrolled Keywords: Ayurveda; bioluminescence; biosensors; Chromobacterium violaceum; lecA::lux; N-acylhomoserine lactones; Pseudomonas aeruginosa; pyocyanin; Syzygium aromaticum; swarming
Subjects: Q Science > QH Natural history
R Medicine
Divisions: Faculty of Medicine
Faculty of Science > Institute of Biological Sciences
Depositing User: Mr. Faizal Hamzah
Date Deposited: 21 Jun 2012 01:29
Last Modified: 30 Oct 2018 01:57
URI: http://eprints.um.edu.my/id/eprint/3377

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