Goh, Neng-Yao and Razif, Muhammad Fazril Mohamad and Yap, Yeannie Hui-Yeng and Ng, Chyan Leong and Fung, Shin-Yee (2022) In silico analysis and characterization of medicinal mushroom cystathionine beta-synthase as an angiotensin converting enzyme (ACE) inhibitory protein. Computational Biology And Chemistry, 96. ISSN 1476-9271, DOI https://doi.org/10.1016/j.compbiolchem.2021.107620.
Full text not available from this repository.Abstract
Angiotensin-converting enzyme (ACE) regulates blood pressure and has been implicated in several conditions including lung injury, fibrosis and Alzheimer's disease. Medicinal mushroom Ganordema lucidum (Reishi) cystathionine beta-synthase (GlCBS) was previously reported to possess ACE inhibitory activities. However, the inhibitory mechanism of CBS protein remains unreported. Therefore, this study integrates in silico sequencing, structural and functional based-analysis, protein modelling, molecular docking and binding affinity calculation to elucidate the inhibitory mechanism of GlCBS and Lignosus rhinocerus (Tiger milk mushroom) CBS protein (LrCBS) towards ACE. In silico analysis indicates that CBSs from both mushrooms share high similarities in terms of physical properties, structural properties and domain distribution. Protein-protein docking analysis revealed that both GlCBS and LrCBS potentially modulate the C-terminal domain of ACE (C-ACE) activity via regulation of chloride activation and/or prevention of substrate entry. GICBS and LrCBS were also shown to interact with ACE at the same region that presumably inhibits the function of ACE.
| Item Type: | Article |
|---|---|
| Funders: | Faculty Research Grant, University of Malaya, Malaysia [GPF003A-2020] [GPF003B-2020] |
| Uncontrolled Keywords: | Cystathionine beta-synthase; Angiotensin-converting enzyme; Ganordema lucidum; Tiger milk mushroom; Lignosus rhinocerus; Protein-protein docking; Chloride blocking; Protein inhibitor |
| Subjects: | R Medicine > R Medicine (General) |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 21 Jul 2022 01:46 |
| Last Modified: | 21 Jul 2022 01:46 |
| URI: | http://eprints.um.edu.my/id/eprint/33625 |
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