Characterization of quorum sensing and quorum quenching soil bacteria isolated from malaysian tropical montane forest

Chong, T.M. and Koh, C.L. and Sam, C.K. and Choo, Y.M. and Yin, W.F. and Chan, Kok Gan (2012) Characterization of quorum sensing and quorum quenching soil bacteria isolated from malaysian tropical montane forest. Sensors, 12 (4). pp. 4846-4859. ISSN 1424-8220, DOI https://doi.org/10.3390/s120404846.

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Official URL: https://doi.org/10.3390/s120404846

Abstract

We report the production and degradation of quorum sensing N-acyl-homoserine lactones by bacteria isolated from Malaysian montane forest soil. Phylogenetic analysis indicated that these isolates clustered closely to the genera of Arthrobacter, Bacillus and Pseudomonas. Quorum quenching activity was detected in six isolates of these three genera by using a series of bioassays and rapid resolution liquid chromatography analysis. Biosensor screening and high resolution liquid chromatography-mass spectrometry analysis revealed the production of N-dodecanoyl-L-homoserine lactone (C12-HSL) by Pseudomonas frederiksbergensis (isolate BT9). In addition to degradation of a wide range of N-acyl-homoserine lactones, Arthrobacter and Pseudomonas spp. also degraded p-coumaroyl-homoserine lactone. To the best of our knowledge, this is the first documentation of Arthrobacter and Pseudomonas spp. capable of degrading p-coumaroyl-homoserine lactone and the production of C12-HSL by P. frederiksbergensis.

Item Type: Article
Funders: UNSPECIFIED
Additional Information: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia; E-Mails: cluster1986@hotmail.com (T.-M.C.); yinwaifong@yahoo.com (W.-F.Y.)
Uncontrolled Keywords: Arthrobacter; Bacillus; liquid chromatography mass spectrometry (LC-MS); N-acylhomoserine lactone; N-dodecanoyl-L-homoserine lactone; p-coumaroylhomoserine lactone; Pseudomonas frederiksbergensis; Quorum quenching; Quorum sensing; Rapid resolution liquid chromatography (RRLC)
Subjects: Q Science > QH Natural history
R Medicine
Divisions: Faculty of Medicine
Faculty of Science > Institute of Biological Sciences
Depositing User: Mr. Faizal Hamzah
Date Deposited: 15 Jun 2012 03:23
Last Modified: 30 Oct 2018 02:00
URI: http://eprints.um.edu.my/id/eprint/3314

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