Lau, Tien-Tien Vicky and Puah, Suat-Moi and Tan, Jin-Ai Mary Anne and Puthucheary, S. D. and Chua, Kek-Heng (2021) Characterization of the relationship between polar and lateral flagellar genes in clinical Aeromonas dhakensis: Phenotypic, genetic and biochemical analyses. Brazilian Journal of Microbiology, 52 (2). pp. 517-529. ISSN 1517-8382, DOI https://doi.org/10.1007/s42770-021-00457-8.
Full text not available from this repository.Abstract
Flagellar-mediated motility is a crucial virulence factor in many bacterial species. A dual flagellar system has been described in aeromonads; however, there is no flagella-related study in the emergent human pathogen Aeromonas dhakensis. Using 46 clinical A. dhakensis, phenotypic motility, genotypic characteristics (flagellar genes and sequence types), biochemical properties and their relationship were investigated in this study. All 46 strains showed swimming motility at 30 degrees C in 0.3% Bacto agar and carried the most prevalent 6 polar flagellar genes cheA, flgE, flgG, flgH, flgL, and flgN. On the contrary, only 18 strains (39%) demonstrated swarming motility on 0.5% Eiken agar at 30 degrees C and they harbored 11 lateral flagellar genes lafB, lafK, lafS, lafT, lafU, flgCL, flgGL, flgNL, fliEL, fliFL, and fliGL. No association was found between biochemical properties and motility phenotypes. Interestingly, a significant association between swarming and strains isolated from pus was observed (p = 0.0171). Three strains 187, 277, and 289 isolated from pus belonged to novel sequence types (ST522 and ST524) exhibited fast swimming and swarming profiles, and they harbored > 90% of the flagellar genes tested. Our findings provide a fundamental understanding of flagellar-mediated motility in A. dhakensis.
| Item Type: | Article |
|---|---|
| Funders: | Universiti Malaya (UMRG RP039B-15HTM) |
| Uncontrolled Keywords: | Aeromonas dhakensis; Flagellar; Lateral; Polar; Swarming; Swimming |
| Subjects: | Q Science > QR Microbiology |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 10 Mar 2022 01:18 |
| Last Modified: | 10 Mar 2022 01:18 |
| URI: | http://eprints.um.edu.my/id/eprint/27841 |
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