Yunan, Nurul Anisa Mat and Shin, Tan Yee and Sabaratnam, Vikineswary (2021) Upcycling the spent mushroom substrate of the grey oyster mushroom pleurotus pulmonarius as a source of lignocellulolytic enzymes for palm oil mill effluent hydrolysis. Journal of Microbiology and Biotechnology, 31 (6). pp. 823-832. ISSN 1017-7825, DOI https://doi.org/10.4014/jmb.2103.03020.
Full text not available from this repository.Abstract
Mushroom cultivation along with the palm oil industry in Malaysia have contributed to large volumes of accumulated lignocellulosic residues that cause serious environmental pollution when these agroresidues are burned. In this study, we illustrated the utilization of lignocellulolytic enzymes from the spent mushroom substrate of Pleurotus pulmonarius for the hydrolysis of palm oil mill effluent (POME). The hydrolysate was used for the production of biohydrogen gas and enzyme assays were carried out to determine the productivities/activities of lignin peroxidase, laccase, xylanase, endoglucanase and beta-glucosidase in spent mushroom substrate. Further, the enzyme cocktails were concentrated for the hydrolysis of POME. Central composite design of response surface methodology was performed to examine the effects of enzyme loading, incubation time and pH on the reducing sugar yield. Productivities of the enzymes for xylanase, laccase, endoglucanase, lignin peroxidase and beta-glucosidase were 2.3, 4.1, 14.6, 214.1, and 915.4 U g(-1), respectively. A maximum of 3.75 g/l of reducing sugar was obtained under optimized conditions of 15 h incubation time with 10% enzyme loading (v/v) at a pH of 4.8, which was consistent with the predicted reducing sugar concentration (3.76 g/l). The biohydrogen cumulative volume (302.78 ml H-2.L-1 POME) and 83.52% biohydrogen gas were recorded using batch fermentation which indicated that the enzymes of spent mushroom substrate can be utilized for hydrolysis of POME.
| Item Type: | Article |
|---|---|
| Funders: | Postgraduate Research Fund, PPP, University of Malaya, Malaysia (PG064-2013B), Ministry of Education Malaysia (MyBrainSc) Scholarship |
| Uncontrolled Keywords: | Pleurotus pulmonarius; Edible mushroom; Enzymatic hydrolysis; Palm oil mill effluent; Spent mushroom substrate; Biohydrogen |
| Subjects: | Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 02 Jun 2022 04:31 |
| Last Modified: | 02 Jun 2022 04:31 |
| URI: | http://eprints.um.edu.my/id/eprint/27233 |
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