Characterization of secreted recombinant Toxoplasma gondii surface antigen 2 (SAG2) heterologously expressed by the yeast Pichia pastoris

Fong, M.Y. and Lau, Y.L. and Zulqarnain, M. (2008) Characterization of secreted recombinant Toxoplasma gondii surface antigen 2 (SAG2) heterologously expressed by the yeast Pichia pastoris. Biotechnology Letters, 30 (4). pp. 611-618. ISSN 0141-5492, DOI https://doi.org/10.1007/s10529-007-9609-x.

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Abstract

The surface antigen 2 (SAG2) gene of the protozoan parasite, Toxoplasma gondii, was cloned and extracellularly expressed in the yeast Pichia pastoris. The effectiveness of the secreted recombinant SAG2 (rSAG2-S) as a serodiagnosis reagent was assessed by western blots and ELISA. In the western blot assay, rSAG2-S reacted with all Toxoplasma-antibody positive human serum samples but not with Toxoplasma-negative samples. In the ELISA, rSAG2-S yielded sensitivity rates ranging from 80 (IgG negative, IgM positive) to 100 (IgG positive, IgM negative). In vivo experiments showed that serum from mice immunized with rSAG2-S reacted specifically with the native SAG2 of T. gondii. These mice were protected when challenged with live cells of T. gondii.

Item Type: Article
Funders: UNSPECIFIED
Additional Information: Fong, Mun Yik Lau, Yee Ling Zulqarnain, Mohamed
Uncontrolled Keywords: Pichia Pastoris; Recombinant SAG2 Antigen; Toxoplasma Gondi
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Prof. Dr. Mun Yik Fong
Date Deposited: 21 Feb 2012 03:46
Last Modified: 14 Jul 2017 08:35
URI: http://eprints.um.edu.my/id/eprint/2723

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