Characterization of secreted recombinant Toxoplasma gondii surface antigen 2 (SAG2) heterologously expressed by the yeast Pichia pastoris

Fong, M.Y. and Lau, Y.L. and Zulqarnain, M. (2008) Characterization of secreted recombinant Toxoplasma gondii surface antigen 2 (SAG2) heterologously expressed by the yeast Pichia pastoris. Biotechnology Letters, 30 (4). pp. 611-618. ISSN 0141-5492, DOI https://doi.org/10.1007/s10529-007-9609-x.

PDF (Parasitology (Medical Parasitology), Diagnostics (Molecular Diagnosis and Recombinant Antigens), Virology (Molecular Characterisation and Epidemiology) ) Characterization_of_secreted_recombinant_Toxoplasma_gondii_surface_antigen_2_(SAG2)_heterologously_expressed_by_the_yeast_Pichia_pastoris.pdf - Published Version Restricted to Registered users only Download (319kB) | Request a copy

Abstract

The surface antigen 2 (SAG2) gene of the protozoan parasite, Toxoplasma gondii, was cloned and extracellularly expressed in the yeast Pichia pastoris. The effectiveness of the secreted recombinant SAG2 (rSAG2-S) as a serodiagnosis reagent was assessed by western blots and ELISA. In the western blot assay, rSAG2-S reacted with all Toxoplasma-antibody positive human serum samples but not with Toxoplasma-negative samples. In the ELISA, rSAG2-S yielded sensitivity rates ranging from 80 (IgG negative, IgM positive) to 100 (IgG positive, IgM negative). In vivo experiments showed that serum from mice immunized with rSAG2-S reacted specifically with the native SAG2 of T. gondii. These mice were protected when challenged with live cells of T. gondii.

Item Type:	Article
Funders:	UNSPECIFIED
Additional Information:	Fong, Mun Yik Lau, Yee Ling Zulqarnain, Mohamed
Uncontrolled Keywords:	Pichia Pastoris; Recombinant SAG2 Antigen; Toxoplasma Gondi
Subjects:	R Medicine
Divisions:	Faculty of Medicine
Depositing User:	Prof. Dr. Mun Yik Fong
Date Deposited:	21 Feb 2012 03:46
Last Modified:	14 Jul 2017 08:35
URI:	http://eprints.um.edu.my/id/eprint/2723

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