Recombinant expression of the larval excretory-secretory antigen TES-120 of Toxocara canis in the methylotrophic yeast Pichia pastoris

Fong, M.Y. and Lau, Y.L. (2004) Recombinant expression of the larval excretory-secretory antigen TES-120 of Toxocara canis in the methylotrophic yeast Pichia pastoris. Parasitology Research, 92 (2). pp. 173-176.

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Abstract

A gene encoding the larval excretory-secretory antigen TES-120 of the dog ascarid worm Toxocara canis was cloned into the methylotrophic yeast Pichia pastoris. Specificity of the recombinant TES-120 antigen produced by the yeast was investigated. Forty-five human serum samples from patients infected with differentparasitic organisms, including 8 cases of toxocariasis, were tested against the recombinant antigen in immunoblot assays. Results from the assays showed that the recombinant TES-120 antigen reacted with sera from toxocariasis patients only. This highly specific recombinant TES-120 antigen can potentially be used for the development of an inexpensive serodiagnostic assay for human toxocariasis.

Item Type:	Article
Funders:	UNSPECIFIED
Additional Information:	M.-Y. Fong (&) Æ Y.-L. Lau Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia E-mail: fongmy@um.edu.my Fax: +60-3-79674754
Uncontrolled Keywords:	Human Toxocariasis
Subjects:	R Medicine
Divisions:	Faculty of Medicine
Depositing User:	Prof. Dr. Mun Yik Fong
Date Deposited:	20 Feb 2012 01:10
Last Modified:	20 Oct 2014 02:57
URI:	http://eprints.um.edu.my/id/eprint/2702

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