Almoustafa, Hassan A. and Alshawsh, Mohammed A. and Chik, Zamri (2021) Targeted polymeric nanoparticle for anthracycline delivery in hypoxia-induced drug resistance in metastatic breast cancer cells. Anti-Cancer Drugs, Publis. ISSN 0959-4973, DOI https://doi.org/10.1097/CAD.0000000000001065.
Full text not available from this repository.Abstract
Poly lactic-co-glycolic acid (PLGA) nanoparticles are intensively studied nanocarriers in drug delivery because of their biodegradability and biochemical characteristics. Polyethylene glycol (PEG) coating for nanocarriers gives them long circulation time in blood and makes them invisible to the reticuloendothelial system. Breast cancer cells have greater uptake of hyaluronic acid compared to normal cells as it binds to their overexpressed CD44 receptors. Since hypoxia plays an important role in cancer metastasis; we formulated PEG-PLGA nanoparticles coated with hyaluronic acid as targeted delivery system for doxorubicin (DOX) using nanoprecipitation method, and characterized them for chemical composition, size, surface charge, shape, and encapsulation efficiency. Then we tested them in vitro on hypoxia-optimized metastatic breast cancer cells. The nanoparticles were spherical with an average size of about 106 +/- 53 nm, a negative surface charge (-15 +/- 3 mV), and high encapsulation efficiency (73.3 +/- 4.1%). In vitro investigation with hypoxia-elevated CD44 MDA-MB-231 cells showed that hyaluronic acid-targeted nanoparticles maintained their efficacy despite hypoxia-induced drug resistance unlike free DOX and nontargeted nanoparticles. In conclusion, this study revealed a simple third generation nanoparticle formulation for targeted treatment of hypoxia-induced drug resistance in breast cancer metastatic cells. Further, optimization is needed including In vivo efficacy and nanoparticle-specific pharmacokinetic studies.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Education, Malaysia (FP031-2014A) |
| Uncontrolled Keywords: | Nanoprecipitation; PEG-PLGA; Hyaluronic acid; Targeted delivery; Breast cancer; Doxorubicin |
| Subjects: | R Medicine R Medicine > RS Pharmacy and materia medica |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 17 Feb 2022 08:16 |
| Last Modified: | 17 Feb 2022 08:18 |
| URI: | http://eprints.um.edu.my/id/eprint/26204 |
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