Ying, Chai Siew and Tahir, Nur Fathiah and Abu Bakar, Nur Syazana and Teo, Chee How and Yusoff, Nur Fatihah Mohd and Baharum, Nadiya Akmal and Saidi, Noor Baity (2020) Genome-wide identification and expression analysis of Banana Rboh genes in response to fusarium oxysporum f. sp. cubense tropical race 4. Malaysian Journal of Biochemistry and Molecular Biology, 23 (2). pp. 46-52. ISSN 1511-2616,
Full text not available from this repository.Abstract
Respiratory burst oxidase homologs (RBOH) is the key enzyme responsible for the production of reactive oxygen species which act as important signal during plant responses to abiotic and biotic stresses. However, RBOH homologs have not been characterized in banana. In this study, we have identified twelve Rboh genes distributed on eight chromosomes of Musa acuminata subsp. malaccensis (DH Pahang) through a genome-wide analysis. MaRbohs exist as sibling paralogs with variable exon-intron structures and highly conserved functional domain. Phylogenetic analysis clustered MaRBOH into four distinct subgroups (I, II, III and IV). The expression of MaRbohs following 24 hours of inoculation with Fusarium oxysporium f. sp. cubense Tropical Race 4 revealed diverse patterns in root tissues. MaRbohH1 and MaRbohA2 were strongly upregulated and downregulated, respectively in response to the root-dip inoculation. This is the first report on genome-wide characterization of Rboh genes from banana and their expression in response to the fungal pathogen TR4. This research provides a basis for exploration of the role of NADPH oxidase in banana defence against the root pathogen. © 2020 Malaysian Society for Biochemistry and Molecular Biology. All rights reserved.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Education Malaysia (FRGS/1/2015/WAB01/UPM/02/11), Universiti Putra Malaysia (GP-IPS/2018/9601900) |
| Uncontrolled Keywords: | Fusarium oxysporum f. sp. cubense Tropical Race 4 (TR4); Musa acuminata cv. Berangan; NADPH oxidase; Respiratory burst oxidase homologs (Rbohs) |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history |
| Divisions: | Deputy Vice Chancellor (Research & Innovation) Office > Centre for Research in Biotechnology for Agriculture |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 18 Feb 2021 04:26 |
| Last Modified: | 18 Feb 2021 04:26 |
| URI: | http://eprints.um.edu.my/id/eprint/25753 |
Actions (login required)
 |
View Item |
