Yu, Shu Pin and Ong, Kien Chai and Tan, Soon Hao and Perera, David and Wong, Kum Thong (2020) Japanese encephalitis virus: Biological clones from a clinical isolate quasispecies show differing neurovirulence in vitro and in a mouse model. Neurology Asia, 25 (3). pp. 279-284. ISSN 1823-6138,
Full text not available from this repository.Abstract
The Japanese encephalitis virus (JEV), a leading cause of encephalitis, exists as quasispecies in clinical isolates. Using a limiting dilution method combined with immunohistochemistry to detect viral antigens, 10 biological clones were isolated and purified from a clinical JEV isolate (CNS138/9) derived from an autopsy brain. These biological clones were tested for neurovirulence in SK-N-MC and NIE-115 neuronal cells, and a 2-week-old, footpad-infected, JE mouse model. Nine clones were found to be neurovirulent; one clone neuroattenuated. Although further studies are needed to determine genotypic differences, if any, in these clones, the limiting dilution purification and neurovirulence testing methods described herein should be useful for phenotypic studies of quasispecies of neurotropic viruses in general, and JEV and other flaviviruses in particular. © 2020, ASEAN Neurological Association. All rights reserved.
| Item Type: | Article |
|---|---|
| Funders: | HIR grant, University Malaya [Project number: UM.C/625/1/HIR/MOHE/MED/06], Postgraduate Research Fund, University Malaya [Project number: PG285-2016A] |
| Uncontrolled Keywords: | Biological clones; Japanese encephalitis virus; Limiting dilution; Quasispecies |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 10 Feb 2021 00:49 |
| Last Modified: | 10 Feb 2021 00:49 |
| URI: | http://eprints.um.edu.my/id/eprint/25742 |
Actions (login required)
 |
View Item |
