Pang, Mei Fong and Tan, Geok Yuan Annie and Abdullah, Noorlidah and Lee, Choon Weng and Ng, Ching Ching (2008) Phylogenetic Analysis of Type I and Type II Polyketide Synthase from Tropical Forest Soil. Biotechnology, 7 (4). pp. 660-668. ISSN 1682-296X, DOI https://doi.org/10.3923/biotech.2008.660.668.
Full text not available from this repository.Abstract
Culture-independent approach was employed to retrieve diverse type I and type II polyketide synthase (PKS) ketosynthase (KS) domains from community DNA extracted from forest topsoil. Type I KS domains detected were from four phyla which comprised Cyanobacteria, Proteobacteria, Actinobacteria, Chloroflexi and uncultured bacteria. The type IIKSα domains were derived from four suborders of actinobacteria in addition to uncultured bacteria. Approximately 25% of the KS domains were recovered from uncultured bacteria and many sequences of type I KS were derived from Myxobacteria. BLASTP results showed that the type I and type II KS domain amino acid sequences were between 52 to 93% identical to the comparative sequences in the GenBank database. Phylogenetic analysis for novelty prediction of KS domains showed 14 and 9 novel clades of type I KS and type II PKS KSα domains, respectively. These phylogenetically distinct novel clades might represent a new subclass of KS domains. Present data suggests the possibility of further discovery of novel genes encoding bioactive compounds which may have medical and pharmaceutical value. © 2008 Asian Network for Scientific Information.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Culture-independent; Ketosynthase (KS); Phylogenetic analysis; Polyketide synthase (PKS); Tropical soil |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 11 Dec 2020 04:20 |
| Last Modified: | 11 Dec 2020 04:20 |
| URI: | http://eprints.um.edu.my/id/eprint/25640 |
Actions (login required)
 |
View Item |
