Ho, Ivan Yee Man and Aziz, Azlina Abdul and Junit, Sarni Mat (2020) Evaluation of Anti-proliferative Effects of Barringtonia racemosa and Gallic Acid on Caco-2 Cells. Scientific Reports, 10 (1). p. 9987. ISSN 2045-2322, DOI https://doi.org/10.1038/s41598-020-66913-x.
Full text not available from this repository.Abstract
Barringtonia racemosa leaf water extract (BLE) had been shown to have high gallic acid (GA) content and BLE has been postulated to have anti-proliferative effects towards colorectal cancer. This study aims to further investigate the mechanism underlying the anti-proliferative effect of BLE in Caco-2 cells and to determine if GA is responsible for the observed effects. Both BLE and GA inhibited Caco-2 cells in a dose-dependent manner. Cells exposed to IC50 concentration of BLE and GA showed reduced antioxidant activities. GA-treated Caco-2 cells experienced higher oxidative stress compared to cells treated with BLE. Both BLE and GA significantly up-regulated the expression of SLC2A1. BLE but not GA, significantly down-regulated the expression of ADH4. Meanwhile, GA but not BLE, significantly up-regulated AKRIB10 and GLO1 but significantly down-regulated HAGH. Alterations in gene expression were coupled with changes in extracellular glucose and pyruvate levels. While BLE decreased intracellular pyruvate, GA did the opposite. Both intracellular and extracellular D-lactate were not affected by either BLE or GA. GA showed more pronounced effects on apoptosis while BLE irreversibly reduced cell percentage in the G0/G1 phase. In conclusion, this study demonstrates the multiple-actions of BLE against Caco-2 cells, potentially involving various polyphenolic compounds, including GA. © 2020, The Author(s).
| Item Type: | Article |
|---|---|
| Funders: | FP015-2013B and FP010-2018A, from the Ministry of Education, Malaysia |
| Uncontrolled Keywords: | Barringtonia Racemosa; Bartogenic Acid; Racemosol |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 04 Aug 2020 03:52 |
| Last Modified: | 04 Aug 2020 03:52 |
| URI: | http://eprints.um.edu.my/id/eprint/25219 |
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