Sohedein, Mohamad Nor Azzimi and Wan-Mohtar, Wan Abd Al Qadr Imad and Yeong, Hui Yin and Ilham, Zul and Chang, Jo-Shu and Supramani, Sugenendran and Phang, Siew Moi (2020) Optimisation of biomass and lipid production of a tropical thraustochytrid Aurantiochytrium sp. UMACC-T023 in submerged-liquid fermentation for large-scale biodiesel production. Biocatalysis and Agricultural Biotechnology, 23. p. 101496. ISSN 1878-8181, DOI https://doi.org/10.1016/j.bcab.2020.101496.
Full text not available from this repository.Abstract
A tropical thraustochytrid, Aurantiochytrium sp. UMACC-T023, was optimised for high biomass and lipid production in submerged-liquid fermentation (SLF). Biomass and lipid production were optimised based on glucose, polypeptone, and yeast extract concentration using response surface methodology (RSM). In RSM, the applied central composite design (CCD) showed that the optimisation model was significant for all variables studied. Yeast extract and polypeptone were associated with significant effects in UMACC-T023 biomass production with p < 0.05. The model was validated by employing the optimised media composition in shake flasks and in 1.4-L stirred-tank bioreactors. The optimised media composition for both biomass and lipid production was 10 g L−1 glucose, 15.08 g L−1 polypeptone, and 13.56 g L−1 yeast extract. Biomass production in the bioreactor increased 2.12-fold compared with the shake flask culture utilising the same optimised media composition. This study demonstrates the potential of large-scale lipid production of UMACC-T023 as biodiesel feedstock. © 2020 Elsevier Ltd
| Item Type: | Article |
|---|---|
| Funders: | Institute of Ocean and Earth Sciences, University of Malaya Research University Grant GA003-2012, RU003E-2017 and RU003I-2017 |
| Uncontrolled Keywords: | Aurantiochytrium sp.; Biodiesel; Biomass; Response surface methodology; Submerged-liquid fermentation |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 17 Jun 2020 02:51 |
| Last Modified: | 17 Jun 2020 02:51 |
| URI: | http://eprints.um.edu.my/id/eprint/24882 |
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