Raghavan, V. (1970) Germination of bracken fern spores. Regulation of protein and RNA synthesis during initiation and growth of the rhizoid. Experimental Cell Research, 63 (2-3). pp. 316-320. ISSN 0014-4827, DOI https://doi.org/10.1016/0014-4827(70)90222-3.
Full text not available from this repository.Abstract
Germination of the spores of bracken fern (Pteridium aquilinum) was accompanied by hydrolysis of storage proteins and increased accumulation of RNA. Incorporation of labeled precursors of protein and RNA into the spores did not begin until about 36 h after sowing. Addition of cycloheximide to the incubation medium reversibly inhibited initiation and growth of the rhizoid and incorporation of 14C-leucine into proteins of the spores. Actinomycin D inhibited elongation of the rhizoid, but not its initiation. RNA synthesis during elongation of the rhizoid was more susceptible to inhibition by actinomycin D than during its initiation. Protein synthesis during initiation and elongation of the rhizoid was not appreciably affected by the drug. Sedimentation profile of RNA on sucrose gradient showed that the only significant incorporation of 3H-uridine during rhizoid initiation was into low molecular weight RNA. There was appreciable incorporation of the label into heavy molecules of RNA during elongation of the rhizoid and actinomycin D completely suppressed this incorporation. The results are discussed in the light of possible mechanism involving the existence of preformed messengers in the dormant spore which provide templates for the first proteins of germination. © 1970.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Additional Information: | Raghavan, V.; School of Biological Sciences, University of Malaya, Malaysia |
| Uncontrolled Keywords: | Carbon Isotopes; Centrifugation, Density Gradient; Cycloheximide; Dactinomycin; Leucine; Methods; Plant Proteins; Plants; RNA; Spectrum Analysis; Spores; Time Factors; Tritium; Uridine |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Mr Jasny Razali |
| Date Deposited: | 12 Mar 2021 02:23 |
| Last Modified: | 12 Mar 2021 02:23 |
| URI: | http://eprints.um.edu.my/id/eprint/24607 |
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