Expression of p53, pRb, Ki-67 and bcl-2 in betel quid induced oral mucosal lesions

Kipli, N.P. and Kumar, S.K.S. and Zain, R.B. and Fan, C.Y. and Boon, P.K. (2005) Expression of p53, pRb, Ki-67 and bcl-2 in betel quid induced oral mucosal lesions. Oral Oncology, 1 (1). p. 87. ISSN 1368-8375, DOI https://doi.org/10.1016/s1744-7895(05)80194-4.

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Abstract

Introduction: Alterations in p53, pRb, KI-67 and bcl-2 have been implicated the development of oral cancer although their combined expression in betel quid induced oral mucosal lesions as not clear. The objective of this study is to investigate the expressions of p53, pRb, K1-67 and bcl-2 m oral mucosa of betel quid chewing subjects. Materials and Methods: A total of 64 tissue sections were obtained from biopsy samples of 50 subjects clinically diagnosed with betel chewer's mucosa (BCM, n=32), BCM with leukoplakia (n=12) and leukoplakia (n=6). Oral squamous cell carcinoma (OSCC, 11=10) and normal oral mucosa (n=4) section were used for comparison. Histopathological analysis was made followed by immunohistochemical detection of p53, pRb, Ka-67 and bcl-2, bcl-2 staining was done 111 25 cases of betel chewer's mucosa with~ or without leukoplakia. Results: Histopathologic analysis revealed atypical hyperplastic changes m 46% and dysplasia in 4% of cases. 28% cases showed p53 expression 111 parabasal and superficial layers of epithelium. 14 cases expressed either abnormal pRb or loss of pRb 111 which 8% of cases had cellular atypia and basal cell hyperplasia. 25 cases (50°,0) expressed K1-67 in superficial layers of epithelium indicating high proliferative activity. 36% (9/25) of cases analysed for bcl-2 showed positivity in basal layers of epithelial. Interestingly, 40% (10/16) cases which were bcl-2 negative had high KI-67 expression. Conclusion: The findings of our study suggest that alterations of p53, pRb, Ka-67 and bcl-2 occur early in oral mucosal lesions of betel quad chewing patients. This may aid in early diagnosis of potentially malignant oral lesions and oral cancer developing 111 the betel quid chewing patients who have neither developed chemically recognizable precursor lesions nor exhibit classical dysplastic changes.

Item Type:	Article
Funders:	UNSPECIFIED
Additional Information:	Prof. Dr. Rosnah Mohd Zain Department of Oral Pathology and Oral Medicine and Periodontology, Faculty of Dentistry, University of Malaya
Uncontrolled Keywords:	Oral squamous cell carcinoma, OSCC, lichenoid lesions, lichen planus, oral cancer, oral tumours, pemphigus, traumatic eosinophilic granuloma, aphthous ulcers, oral mucosal lesions, betel chewers mucosa, betel quid related lesions, betel quid, areca quid, tobacco quid, oral cancer screening, training and calibration, early detection, oral cancer awareness, biobanking, tissue bank, databank, oral cancer, tissue bank, research credibility, research ethics.
Subjects:	R Medicine > RK Dentistry > Oral surgery
Divisions:	Faculty of Dentistry > Dept of Oral & Maxillofacial Surgery
Depositing User:	Prof. Dr. Rosnah Mohd Zain
Date Deposited:	17 Jan 2012 01:45
Last Modified:	13 Nov 2019 02:32
URI:	http://eprints.um.edu.my/id/eprint/2447

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