Lee, Soo Leng and Rahman, Zainal Ariff Abdul and Tsujigiwa, Hidetsugu and Hamada, Mei and Takabatake, Kiyofumi and Nakano, Keisuke and Nagatsuka, Hitoshi and Siar, Chong Huat (2019) An in vitro Three-Dimensional Co-Culture System for Ameloblastoma Modelling (Sistem Ko-Kultur Tiga Dimensi secara in vitro untuk Pemodelan Ameloblastoma). Sains Malaysiana, 48 (8). pp. 1697-1706. ISSN 0126-6039, DOI https://doi.org/10.17576/jsm-2019-4808-15.
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Abstract
Ameloblastoma, the most clinically significant odontogenic epithelial tumor, is a locally-invasive and destructive lesion in the jawbones. However, the nature of this infiltrativeness and destructive behavior remains ill-understood. To address this, we established an in vitro three-dimensional (3D) co-culture system to simulate an amelobastoma disease model aimed at investigating the interactions between tumor cells and osteoblasts. Osteoblastic cell lines (KUSA/A1 and mc3T3-E1) and one stromal cell line (ST2) were separately co-seeded with ameloblastoma-derived cell line (AM-1) in a collagen scaffold (representing the extracellular bone matrix) and incubated with mineralization medium. Inununohistochemistry, double immunofluorescence and mineralization assay were performed. Only Am-1/KUsA-A1 co-culture showed a significant increase in Am-1 cell count, suggesting that heterotypic cell-cell interaction promotes tumoral cell growth, while formation of visible Am-1 epithelial nest-like structures resembling ameloblastoma cells in their native state, suggest morphodffferentiation. A RANK-high, RANKL-low and osteoprotegerin-low immunoprofile in co-culture AM-1 cells implies deregulated osteoclastogenesis. Mineralization assays showed diminished calcification in Am-1/KUSA-A1 co-culture extracellular matrix suggesting an altered local bone metabolism. In contrast, KUSA/A1 monocultures showed abundant extracellular matrix calcification. Taken together, these results suggest that a 3D co-culture system as an amelobastoma disease model provides insights that bidirectional ameloblastoma-osteoblastic interactions might play a role in modulating tumor growth and osteoclastogenesis.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Ameloblast; ameloblastoma modelling; co-culture system; pre-osteoblast |
| Subjects: | R Medicine R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer) |
| Divisions: | Faculty of Dentistry > Dept of Oral & Maxillofacial Surgery |
| Depositing User: | Mr Ahmad Azwan Azman |
| Date Deposited: | 21 Feb 2020 04:01 |
| Last Modified: | 21 Feb 2020 04:01 |
| URI: | http://eprints.um.edu.my/id/eprint/23888 |
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