Puah, Suat Moi and Puthucheary, Savithri Devi and Chua, Kek Heng (2019) First report of extended-spectrum β-lactamases TEM-116 and OXA-10 in clinical isolates of alcaligenes species from Kuala Lumpur, Malaysia. Japanese Journal of Infectious Diseases, 72 (4). pp. 266-269. ISSN 1344-6304, DOI https://doi.org/10.7883/yoken.JJID.2018.031.
Full text not available from this repository.Abstract
There is an alarming increase in the prevalence of extended-spectrum β-lactamases (ESBLs) present mainly in Enterobacteriaceae and other nonfermenting gram-negative bacteria, such as Alcaligenes faecalis, which is the only species in that genus that is clinically relevant. We investigated Alcaligenes species from 7 cases (6 inpatients and one outpatient) at our tertiary-care hospital. Four patients had urinary tract infections, and one each had systemic lupus erythematosus, pulmonary stenosis, and diabetic ulcer. All 7 isolates were identified as Alcaligenes spp. based on their 16S rRNA gene sequences, and antibiotic susceptibility was determined using a Vitek 2 system with AST-GN87 cards. All the strains were resistant to cefazolin; 6 were resistant to trimethoprim/sulfamethoxazole; 5 manifested resistance to ampicillin/sulbactam, cefepime, tobramycin, ciprofloxacin, and nitrofurantoin; whereas 5 had multidrug resistance profiles. All the strains (7/7) expressed ESBL activity; PCR screening and sequencing showed evidence of genes blaTEM-116 (7/7) and blaOXA-10 (4/7), and we believe that this is the first report on the presence of TEM-116 and OXA-10 in an Alcaligenes spp. A combination of the 2 genes was present in 4 strains. All 7 strains were found to harbor at least one ESBL gene probably contributing to the drug resistance. © 2019, National Institute of Health. All rights reserved.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Beta-Lactamases; Klebsiella pneumoniae; Extended-spectrum β-lactamase |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 11 Feb 2020 03:56 |
| Last Modified: | 11 Feb 2020 03:56 |
| URI: | http://eprints.um.edu.my/id/eprint/23738 |
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